Structure-Dependent Binding of hnRNPA1 to Telomere RNA

Xiao Liu, Takumi Ishizuka, Hong Liang Bao, Kei Wada, Yuma Takeda, Keisuke Iida, Kazuo Nagasawa, Danzhou Yang, Yan Xu

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Telomeric repeat-containing RNA is a new noncoding RNA molecule that performs various biofunctions. Heterogeneous nuclear ribonucleoprotein (hnRNP) A1 is an RNA-binding protein involved in the telomere maintenance machinery. To date, little is known about how hnRNPA1 binds to telomeric RNA. In this study, we investigated the binding affinity and recognition mechanism of telomere RNA with the RNA recognition motif of hnRNPA1. Using the photochemical cross-linking method, we showed that the telomere RNA G-quadruplex with loops is important in the interaction of telomere RNA with hnRNPA1. Using small-molecule probes, we directly visualized the complex formed by the telomere RNA G-quadruplex and hnRNPA1 in vitro and in live cells. The results suggested that the structure-dependent binding of hnRNPA1 to telomere RNA regulates the telomere function. Therefore, our study provides new insights into the interactions between the RNA G-quadruplex and proteins at the telomere.

Original languageEnglish (US)
Pages (from-to)7533-7539
Number of pages7
JournalJournal of the American Chemical Society
Volume139
Issue number22
DOIs
StatePublished - Jun 7 2017
Externally publishedYes

Fingerprint

Telomere
RNA
G-Quadruplexes
Heterogeneous-Nuclear Ribonucleoproteins
Untranslated RNA
RNA-Binding Proteins
Molecules
GTP-Binding Proteins
Machinery
Maintenance
Proteins

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

Cite this

Liu, X., Ishizuka, T., Bao, H. L., Wada, K., Takeda, Y., Iida, K., ... Xu, Y. (2017). Structure-Dependent Binding of hnRNPA1 to Telomere RNA. Journal of the American Chemical Society, 139(22), 7533-7539. https://doi.org/10.1021/jacs.7b01599

Structure-Dependent Binding of hnRNPA1 to Telomere RNA. / Liu, Xiao; Ishizuka, Takumi; Bao, Hong Liang; Wada, Kei; Takeda, Yuma; Iida, Keisuke; Nagasawa, Kazuo; Yang, Danzhou; Xu, Yan.

In: Journal of the American Chemical Society, Vol. 139, No. 22, 07.06.2017, p. 7533-7539.

Research output: Contribution to journalArticle

Liu, X, Ishizuka, T, Bao, HL, Wada, K, Takeda, Y, Iida, K, Nagasawa, K, Yang, D & Xu, Y 2017, 'Structure-Dependent Binding of hnRNPA1 to Telomere RNA', Journal of the American Chemical Society, vol. 139, no. 22, pp. 7533-7539. https://doi.org/10.1021/jacs.7b01599
Liu X, Ishizuka T, Bao HL, Wada K, Takeda Y, Iida K et al. Structure-Dependent Binding of hnRNPA1 to Telomere RNA. Journal of the American Chemical Society. 2017 Jun 7;139(22):7533-7539. https://doi.org/10.1021/jacs.7b01599
Liu, Xiao ; Ishizuka, Takumi ; Bao, Hong Liang ; Wada, Kei ; Takeda, Yuma ; Iida, Keisuke ; Nagasawa, Kazuo ; Yang, Danzhou ; Xu, Yan. / Structure-Dependent Binding of hnRNPA1 to Telomere RNA. In: Journal of the American Chemical Society. 2017 ; Vol. 139, No. 22. pp. 7533-7539.
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