Structure function analysis of the H-2 Abp gene

Jonathan A. Harton, Wayne Litaker, Jeffrey A Frelinger, Gail A. Bishop

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The gene encoding the H-2 Ap class II β chain was isolated from a B10.P genomic library and sequenced. This gene was also used to construct transfectants of the CH12 lymphoma clone CH12.LX, which express the Abp gene product in association with the endogenous Aαk chain. We present here the first report of the complete nucleotide coding sequence of Abp. The predicted amino acid sequence of Abp reveals only five residues different from Ab q, four of which are present in the mature peptide. These four amino acid changes could account for the differential susceptibility of H-2 q vs H-2 p mice to the development of collagen-induced arthritis (CIA). Antibodies specific for the transfected Abp protein induce CH 12. LX cells to secrete immunoglobulin in the presence of antigen. Comparison of the amino acid sequence with other Aβ chains that have been tested in signal transduction experiments suggests that amino acid 9 may be important to the signaling ability of class II A molecules.

Original languageEnglish (US)
Pages (from-to)358-365
Number of pages8
JournalImmunogenetics
Volume34
Issue number6
DOIs
StatePublished - Nov 1991
Externally publishedYes

Fingerprint

Amino Acid Sequence
Genes
Amino Acids
Experimental Arthritis
Genomic Library
Immunoglobulins
Signal Transduction
Lymphoma
Clone Cells
Antigens
Peptides
Antibodies
Proteins

ASJC Scopus subject areas

  • Immunology
  • Genetics

Cite this

Structure function analysis of the H-2 Abp gene. / Harton, Jonathan A.; Litaker, Wayne; Frelinger, Jeffrey A; Bishop, Gail A.

In: Immunogenetics, Vol. 34, No. 6, 11.1991, p. 358-365.

Research output: Contribution to journalArticle

Harton, JA, Litaker, W, Frelinger, JA & Bishop, GA 1991, 'Structure function analysis of the H-2 Abp gene', Immunogenetics, vol. 34, no. 6, pp. 358-365. https://doi.org/10.1007/BF01787485
Harton, Jonathan A. ; Litaker, Wayne ; Frelinger, Jeffrey A ; Bishop, Gail A. / Structure function analysis of the H-2 Abp gene. In: Immunogenetics. 1991 ; Vol. 34, No. 6. pp. 358-365.
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