Structure of a viral procapsid with molecular scaffolding

Michael G. Rossmann, Terje Dokland, Ricardo Bemal, Robert McKenna, Leodevico L. Dag, Bentley A Fane

Research output: Contribution to journalArticle

Abstract

Scaffolding proteins often play a catalytic role in the assembly process, rather like molecular chaperones. Although macromolecular assembly processes are fundamental to all biological systems, they have been characterized most thoroughly in viral systems, such as the icosahedral Eschcrichia coli bacteriophage 4X174. The XI74 virion contains the proteins F, G, H and I. During assembly, two scaffolding proteins B and D are required for the formation of a 108S, 360-A diameter procapsid from pentameric precursors containing the F, G and H proteins. The procapsid contains 240 copies of protein D, forming an external scaffold, and 60 copies each of the internal scaffolding protein B, the capsid protein F, and the spike protein G. Maturadon involves packaging of DNA andJ proteins and loss of protein B, producing a 132S intermediate. Subsequent removal of the external scaffold yields the mature virion. Both the F and G proteins have the eight-stranded antiparallel -sandwich motif common to many plant and animal viruses. The structure of a procapsid-like particle at 3.5 A resolution will be described, showing how the scaffolding proteins coordinate assembly of the virus by interactions with the F and G proteins, and the F protein undergoes conformational changes during capsid maturation.

Original languageEnglish (US)
JournalFASEB Journal
Volume12
Issue number8
StatePublished - 1998
Externally publishedYes

Fingerprint

viral morphology
Viral Structures
scaffolding proteins
Proteins
proteins
G-proteins
virion
Viruses
GTP-Binding Proteins
Scaffolds
Virion
molecular chaperones
protein depletion
capsid
plant viruses
DNA Packaging
sandwiches
coat proteins
Virus Assembly
Plant Viruses

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Rossmann, M. G., Dokland, T., Bemal, R., McKenna, R., Dag, L. L., & Fane, B. A. (1998). Structure of a viral procapsid with molecular scaffolding. FASEB Journal, 12(8).

Structure of a viral procapsid with molecular scaffolding. / Rossmann, Michael G.; Dokland, Terje; Bemal, Ricardo; McKenna, Robert; Dag, Leodevico L.; Fane, Bentley A.

In: FASEB Journal, Vol. 12, No. 8, 1998.

Research output: Contribution to journalArticle

Rossmann, MG, Dokland, T, Bemal, R, McKenna, R, Dag, LL & Fane, BA 1998, 'Structure of a viral procapsid with molecular scaffolding', FASEB Journal, vol. 12, no. 8.
Rossmann MG, Dokland T, Bemal R, McKenna R, Dag LL, Fane BA. Structure of a viral procapsid with molecular scaffolding. FASEB Journal. 1998;12(8).
Rossmann, Michael G. ; Dokland, Terje ; Bemal, Ricardo ; McKenna, Robert ; Dag, Leodevico L. ; Fane, Bentley A. / Structure of a viral procapsid with molecular scaffolding. In: FASEB Journal. 1998 ; Vol. 12, No. 8.
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