Structure of the copper sites in membrane-bound cytochrome c oxidase.

Linda S Powers, B. Chance, Y. C. Ching, C. P. Lee

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The structures of membrane proteins are difficult to obtain by crystallography and may be altered by the detergents used in their extraction. X-ray absorption spectroscopy has been used to identify the structures of the copper atoms of the membrane-bound enzyme in mitochondria and in submitochondrial particles at respective concentrations of 100 and 200 micron of molar copper. To within the experimental error, the x-ray absorption spectra of the copper atoms of the membrane-bound and the Yonetani (Yonetani, T. (1961) J. Biol. Chem. 236, 1680-1688) purified oxidase are identical; all detectable shells of the active site indicate no alteration of structural parameters. Significant differences are found when compared to the Hartzell-Beinert (Hartzell, R. C., and Beinert, H. (1974) Biochim. Biophys. Acta 368, 318-338) preparation. Extended x-ray absorption fine structure technology is now adequate for the direct studies of membrane proteins in situ in their natural environment.

Original languageEnglish (US)
Pages (from-to)3160-3164
Number of pages5
JournalJournal of Biological Chemistry
Volume262
Issue number7
StatePublished - Mar 5 1987
Externally publishedYes

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Electron Transport Complex IV
Copper
Membranes
Membrane Proteins
X-Ray Absorption Spectroscopy
X-Rays
Submitochondrial Particles
X rays
Atoms
Crystallography
Mitochondria
X ray absorption spectroscopy
Detergents
Absorption spectra
Catalytic Domain
Oxidoreductases
Technology
Enzymes

ASJC Scopus subject areas

  • Biochemistry

Cite this

Structure of the copper sites in membrane-bound cytochrome c oxidase. / Powers, Linda S; Chance, B.; Ching, Y. C.; Lee, C. P.

In: Journal of Biological Chemistry, Vol. 262, No. 7, 05.03.1987, p. 3160-3164.

Research output: Contribution to journalArticle

Powers, LS, Chance, B, Ching, YC & Lee, CP 1987, 'Structure of the copper sites in membrane-bound cytochrome c oxidase.', Journal of Biological Chemistry, vol. 262, no. 7, pp. 3160-3164.
Powers, Linda S ; Chance, B. ; Ching, Y. C. ; Lee, C. P. / Structure of the copper sites in membrane-bound cytochrome c oxidase. In: Journal of Biological Chemistry. 1987 ; Vol. 262, No. 7. pp. 3160-3164.
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