Structures, functions, and mechanisms of filament forming enzymes: a renaissance of enzyme filamentation

Chad K. Park, Nancy C. Horton

Research output: Contribution to journalReview article

2 Scopus citations

Abstract

Filament formation by non-cytoskeletal enzymes has been known for decades, yet only relatively recently has its wide-spread role in enzyme regulation and biology come to be appreciated. This comprehensive review summarizes what is known for each enzyme confirmed to form filamentous structures in vitro, and for the many that are known only to form large self-assemblies within cells. For some enzymes, studies describing both the in vitro filamentous structures and cellular self-assembly formation are also known and described. Special attention is paid to the detailed structures of each type of enzyme filament, as well as the roles the structures play in enzyme regulation and in biology. Where it is known or hypothesized, the advantages conferred by enzyme filamentation are reviewed. Finally, the similarities, differences, and comparison to the SgrAI endonuclease system are also highlighted.

Original languageEnglish (US)
Pages (from-to)927-994
Number of pages68
JournalBiophysical Reviews
Volume11
Issue number6
DOIs
Publication statusPublished - Dec 1 2019

    Fingerprint

Keywords

  • DNA binding
  • Enzyme
  • Nuclease
  • Regulation
  • Run-on oligomerization
  • Self-association

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Molecular Biology

Cite this