Studies of tyrosine phosphorylation and Src family tyrosine kinases in the lens epithelium

Shigeo Tamiya, Nicholas A. Delamere

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

PURPOSE. Tyrosine phosphorylation regulates many aspects of cell function; thus, cells that have different roles often display different patterns of tyrosine phosphorylation. Because there is interest in differential function of the anterior and equatorial regions of the lens epithelium, studies were conducted to compare tyrosine phosphorylation in the two zones. METHODS. Anterior and equatorial regions of the porcine lens epithelium were collected. Using Western blot analysis, tissue homogenates were probed for tyrosine kinase proteins, phospho-Src, phosphotyrosine, and proliferating cell nuclear antigen (PCNA). RESULTS. Phosphotyrosine immunoblots revealed a marked difference between the pattern of tyrosine phosphorylation in anterior and equatorial regions of the epithelium. Many more bands were detected in the equatorial region, and band density was greater. The abundance of total and active Src family kinases was higher at the equator than at the anterior epithelium. Src kinase activity, which was measured directly by quantifying phosphorylation of a synthetic target peptide using 32P-γ-ATP, was detectable only at the equator. In organ-cultured lenses, PP2, a specific inhibitor of the Src kinase family, reduced the density of the phosphotyrosine protein bands. The abundance of PCNA, a protein expressed in proliferating cells, also was reduced in PP2-treated lenses. CONCLUSIONS. The results suggest that the higher Src family kinase activity at the equator contributes to the higher degree of protein phosphorylation observed in this region. The ability of PP2 to suppress PCNA expression suggests a possible link between the activity of Src family kinases and cell proliferation.

Original languageEnglish (US)
Pages (from-to)2076-2081
Number of pages6
JournalInvestigative Ophthalmology and Visual Science
Volume46
Issue number6
DOIs
StatePublished - Jun 1 2005
Externally publishedYes

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

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