Study design and sample sizes for a lacI transgenic mouse mutation assay

Walter W Piegorsch, B. H. Margolin, M. D. Shelby, A. Johnson, J. E. French, R. W. Tennant, K. R. Tindall

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

Design features that adjust and account for excess variation in a transgenic mouse mutation assay based on a lacl target transgene from E. coli are considered. These features include proper identification of plate, packaging reaction, and animal identifier codes throughout the experimental and analysis phases of the study, 'blocking' of exposed and unexposed animals when preparing and plating multiple packaging reactions from the same genomic DNA sample, separating sectored mutant plaques and complete mutant plaques before performing any quantitative analyses, and testing for sources of excess variation attributable to features of the experimental protocol - such as plate-to-plate (within packaging reactions), packaging reaction-to-packaging reaction (within animals), and animal-to-animal (within study). Control and ethylnitrosourea-treated animal data are presented from a fully designed study in the lacl assay. The study design incorporates many of these experimental principles. Statistical methods to identify excess variability are noted, and the designed study data are used to illustrate the types of variability encountered in practice. A standard statistical test for two-sample testing is highlighted, from which recommendations are made for sample size selection in future studies.

Original languageEnglish (US)
Pages (from-to)231-245
Number of pages15
JournalEnvironmental and Molecular Mutagenesis
Volume25
Issue number3
DOIs
StatePublished - 1995
Externally publishedYes

Fingerprint

Sample Size
Transgenic Mice
Product Packaging
mutation
Assays
Animals
assay
Packaging
Mutation
animal
Ethylnitrosourea
size selection
Statistical tests
Testing
Transgenes
Plating
Escherichia coli
Statistical methods
genomics
packaging

Keywords

  • Experiment design
  • Extra-binomial varibility
  • Hierarchical sampling
  • Mutagenicity
  • Sample size determinations
  • Sectored plaques
  • Statistical methods
  • Transgene

ASJC Scopus subject areas

  • Environmental Science(all)
  • Environmental Chemistry
  • Genetics
  • Genetics(clinical)
  • Toxicology
  • Health, Toxicology and Mutagenesis

Cite this

Piegorsch, W. W., Margolin, B. H., Shelby, M. D., Johnson, A., French, J. E., Tennant, R. W., & Tindall, K. R. (1995). Study design and sample sizes for a lacI transgenic mouse mutation assay. Environmental and Molecular Mutagenesis, 25(3), 231-245. https://doi.org/10.1002/em.2850250310

Study design and sample sizes for a lacI transgenic mouse mutation assay. / Piegorsch, Walter W; Margolin, B. H.; Shelby, M. D.; Johnson, A.; French, J. E.; Tennant, R. W.; Tindall, K. R.

In: Environmental and Molecular Mutagenesis, Vol. 25, No. 3, 1995, p. 231-245.

Research output: Contribution to journalArticle

Piegorsch, WW, Margolin, BH, Shelby, MD, Johnson, A, French, JE, Tennant, RW & Tindall, KR 1995, 'Study design and sample sizes for a lacI transgenic mouse mutation assay', Environmental and Molecular Mutagenesis, vol. 25, no. 3, pp. 231-245. https://doi.org/10.1002/em.2850250310
Piegorsch, Walter W ; Margolin, B. H. ; Shelby, M. D. ; Johnson, A. ; French, J. E. ; Tennant, R. W. ; Tindall, K. R. / Study design and sample sizes for a lacI transgenic mouse mutation assay. In: Environmental and Molecular Mutagenesis. 1995 ; Vol. 25, No. 3. pp. 231-245.
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