Study of the Expression Transition of Cardiac Myosin Using Polarization-Dependent SHG Microscopy

Cai Yuan, Xiaolei Zhao, Zhonghai Wang, Thomas K. Borg, Tong Ye, Zain I. Khalpey, Raymond B. Runyan, Yonghong Shao, Bruce Z. Gao

Research output: Contribution to journalArticle

Abstract

Detection of the transition between the two myosin isoforms α- and β-myosin in living cardiomyocytes is essential for understanding cardiac physiology and pathology. In this study, the differences in symmetry of polarization spectra obtained from α- and β-myosin in various mammalian ventricles and propylthiouracil-treated rats are explored through polarization-dependent second harmonic generation microscopy. Here, we report for the, to our knowledge, first time that α- and β-myosin, as protein crystals, possess different symmetries: the former has C6 symmetry, and the latter has C3v. A single-sarcomere line scan further demonstrated that the differences in polarization-spectrum symmetry between α- and β-myosin came from their head regions: the head and neck domains of α- and β-myosin account for the differences in symmetry. In addition, the dynamic transition of the polarization spectrum from C6 to C3v line profile was observed in a cell culture in which norepinephrine induced an α- to β-myosin transition.

Original languageEnglish (US)
Pages (from-to)1058-1066
Number of pages9
JournalBiophysical Journal
Volume118
Issue number5
DOIs
StatePublished - Mar 10 2020

ASJC Scopus subject areas

  • Biophysics

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    Yuan, C., Zhao, X., Wang, Z., Borg, T. K., Ye, T., Khalpey, Z. I., Runyan, R. B., Shao, Y., & Gao, B. Z. (2020). Study of the Expression Transition of Cardiac Myosin Using Polarization-Dependent SHG Microscopy. Biophysical Journal, 118(5), 1058-1066. https://doi.org/10.1016/j.bpj.2019.12.030