Studying chemoattractant signal transduction dynamics in Dictyostelium by BRET

A. F M Tariqul Islam, Branden M. Stepanski, Pascale G Charest

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations

Abstract

Understanding the dynamics of chemoattractant signaling is key to our understanding of the mechanisms underlying the directed migration of cells, including that of neutrophils to sites of infections and of cancer cells during metastasis. A model frequently used for deciphering chemoattractant signal transduction is the social amoeba Dictyostelium discoideum. However, the methods available to quantitatively measure chemotactic signaling are limited. Here, we describe a protocol to quantitatively study chemoattractant signal transduction in Dictyostelium by monitoring protein-protein interactions and conformational changes using Bioluminescence Resonance Energy Transfer (BRET).

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages63-77
Number of pages15
Volume1407
DOIs
StatePublished - 2016

Publication series

NameMethods in Molecular Biology
Volume1407
ISSN (Print)10643745

Keywords

  • Bioluminescence resonance energy transfer
  • Chemotaxis
  • Dictyostelium discoideum
  • Directed cell migration
  • GPCR
  • Heterotrimeric G proteins
  • Protein-protein interaction
  • Signaling dynamics

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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    Islam, A. F. M. T., Stepanski, B. M., & Charest, P. G. (2016). Studying chemoattractant signal transduction dynamics in Dictyostelium by BRET. In Methods in Molecular Biology (Vol. 1407, pp. 63-77). (Methods in Molecular Biology; Vol. 1407). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-3480-5_5