Subarachnoid Hemorrhage Induces Gliosis and Increased Expression of the Pro-inflammatory Cytokine High Mobility Group Box 1 Protein

Kentaro Murakami, Masayo Koide, Travis M Dumont, Sheila R. Russell, Bruce I. Tranmer, George C. Wellman

Research output: Contribution to journalArticle

69 Citations (Scopus)

Abstract

Subarachnoid hemorrhage (SAH) following cerebral aneurysm rupture is associated with high rates of morbidity and mortality. Surviving SAH patients often suffer from neurological impairment, yet little is currently known regarding the influence of subarachnoid blood on brain parenchyma. The objective of the present study was to examine the impact of subarachnoid blood on glial cells using a rabbit SAH model. The astrocyte-specific proteins, glial fibrillary acidic protein (GFAP) and S100B, were upregulated in brainstem from SAH model rabbits, consistent with the development of reactive astrogliosis. In addition to reactive astrogliosis, cytosolic expression of the proinflammatory cytokine, high-mobility group box 1 protein (HMGB1) was increased in brain from SAH animals. We found that greater than 90% of cells expressing cytosolic HMGB1 immunostained positively for Iba1, a specific marker for microglia and macrophages. Further, the number of Iba1-positive cells was similar in brain from control and SAH animals, suggesting the majority of these cells were likely resident microglial cells rather than infiltrating macrophages. These observations demonstrate SAH impacts brain parenchyma by activating astrocytes and microglia, triggering up-regulation of the pro-inflammatory cytokine HMGB1.

Original languageEnglish (US)
Pages (from-to)72-79
Number of pages8
JournalTranslational Stroke Research
Volume2
Issue number1
DOIs
StatePublished - Mar 2011
Externally publishedYes

Fingerprint

HMGB1 Protein
Gliosis
Subarachnoid Hemorrhage
Cytokines
Intracranial Hemorrhages
Microglia
Astrocytes
Macrophages
Rabbits
Glial Fibrillary Acidic Protein
Brain
Intracranial Aneurysm
Neuroglia
Brain Stem
Rupture
Up-Regulation
Morbidity
Mortality

Keywords

  • Inflammation
  • Microglia
  • Reactive astrogliosis
  • Subarachnoid hemorrhage
  • Vasospasm

ASJC Scopus subject areas

  • Clinical Neurology
  • Neuroscience(all)
  • Cardiology and Cardiovascular Medicine

Cite this

Subarachnoid Hemorrhage Induces Gliosis and Increased Expression of the Pro-inflammatory Cytokine High Mobility Group Box 1 Protein. / Murakami, Kentaro; Koide, Masayo; Dumont, Travis M; Russell, Sheila R.; Tranmer, Bruce I.; Wellman, George C.

In: Translational Stroke Research, Vol. 2, No. 1, 03.2011, p. 72-79.

Research output: Contribution to journalArticle

Murakami, Kentaro ; Koide, Masayo ; Dumont, Travis M ; Russell, Sheila R. ; Tranmer, Bruce I. ; Wellman, George C. / Subarachnoid Hemorrhage Induces Gliosis and Increased Expression of the Pro-inflammatory Cytokine High Mobility Group Box 1 Protein. In: Translational Stroke Research. 2011 ; Vol. 2, No. 1. pp. 72-79.
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