Substance P and substance K receptor binding sites in the human gastrointestinal tract: Localization by autoradiography

T. S. Gates, R. P. Zimmerman, C. R. Mantyh, S. R. Vigna, J. E. Maggio, M. L. Welton, E. P. Passaro, Patrick W Mantyh

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

Quantitative receptor autoradiography was used to localize and quantify the distribution of binding sites for 125I-radiolabeled substance P (SP), substance K (SK) and neuromedin K (NK) in the human GI tract using histologically normal tissue obtained from uninvolved margins of resections for carcinoma. The distribution of SP and SK binding sites is different for each gastrointestinal (GI) segment examined. Specific SP binding sites are expressed by arterioles and venules, myenteric plexus, external circular muscle, external longitudinal muscle, muscularis mucosa, epithelial cells of the mucosa, and the germinal centers of lymph nodules. SK binding sites are distributed in a pattern distinct from SP binding sites and are localized to the external circular muscle, external longitudinal muscle, and the muscularis mucosa. Binding sites for NK were not detected in any part of the human GI tract. These results demonstrate that: 1) surgical specimens from the human GI tract can be effectively processed for quantitative receptor autoradiography; 2) of the three mammalian tachykinins tested, SP and SK, but not NK binding sites are expressed in detectable levels in the human GI tract; 3) whereas SK receptor binding sites are expressed almost exclusively by smooth muscle, SP binding sites are expressed by smooth muscle cells, arterioles, venules, epithelial cells of the mucosa and cells associated with lymph nodules; and 4) both SP and SK binding sites expressed by smooth muscle are more stable than SP binding sites expressed by blood vessels, lymph nodules, and mucosal cells.

Original languageEnglish (US)
Pages (from-to)1207-1219
Number of pages13
JournalPeptides
Volume9
Issue number6
DOIs
StatePublished - 1988
Externally publishedYes

Fingerprint

Neurokinin-2 Receptors
Substance P
Autoradiography
Gastrointestinal Tract
Binding Sites
Neurokinin A
Muscle
Neurokinin B
Mucous Membrane
Lymph
Muscles
Venules
Arterioles
Smooth Muscle
Epithelial Cells
Tachykinins
Myenteric Plexus
Germinal Center
Blood vessels
Smooth Muscle Myocytes

Keywords

  • Gastrointestinal tract
  • Receptor
  • Substance K
  • Substance P
  • Tachykinins

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Physiology
  • Cellular and Molecular Neuroscience

Cite this

Substance P and substance K receptor binding sites in the human gastrointestinal tract : Localization by autoradiography. / Gates, T. S.; Zimmerman, R. P.; Mantyh, C. R.; Vigna, S. R.; Maggio, J. E.; Welton, M. L.; Passaro, E. P.; Mantyh, Patrick W.

In: Peptides, Vol. 9, No. 6, 1988, p. 1207-1219.

Research output: Contribution to journalArticle

Gates, TS, Zimmerman, RP, Mantyh, CR, Vigna, SR, Maggio, JE, Welton, ML, Passaro, EP & Mantyh, PW 1988, 'Substance P and substance K receptor binding sites in the human gastrointestinal tract: Localization by autoradiography', Peptides, vol. 9, no. 6, pp. 1207-1219. https://doi.org/10.1016/0196-9781(88)90184-2
Gates, T. S. ; Zimmerman, R. P. ; Mantyh, C. R. ; Vigna, S. R. ; Maggio, J. E. ; Welton, M. L. ; Passaro, E. P. ; Mantyh, Patrick W. / Substance P and substance K receptor binding sites in the human gastrointestinal tract : Localization by autoradiography. In: Peptides. 1988 ; Vol. 9, No. 6. pp. 1207-1219.
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T2 - Localization by autoradiography

AU - Gates, T. S.

AU - Zimmerman, R. P.

AU - Mantyh, C. R.

AU - Vigna, S. R.

AU - Maggio, J. E.

AU - Welton, M. L.

AU - Passaro, E. P.

AU - Mantyh, Patrick W

PY - 1988

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N2 - Quantitative receptor autoradiography was used to localize and quantify the distribution of binding sites for 125I-radiolabeled substance P (SP), substance K (SK) and neuromedin K (NK) in the human GI tract using histologically normal tissue obtained from uninvolved margins of resections for carcinoma. The distribution of SP and SK binding sites is different for each gastrointestinal (GI) segment examined. Specific SP binding sites are expressed by arterioles and venules, myenteric plexus, external circular muscle, external longitudinal muscle, muscularis mucosa, epithelial cells of the mucosa, and the germinal centers of lymph nodules. SK binding sites are distributed in a pattern distinct from SP binding sites and are localized to the external circular muscle, external longitudinal muscle, and the muscularis mucosa. Binding sites for NK were not detected in any part of the human GI tract. These results demonstrate that: 1) surgical specimens from the human GI tract can be effectively processed for quantitative receptor autoradiography; 2) of the three mammalian tachykinins tested, SP and SK, but not NK binding sites are expressed in detectable levels in the human GI tract; 3) whereas SK receptor binding sites are expressed almost exclusively by smooth muscle, SP binding sites are expressed by smooth muscle cells, arterioles, venules, epithelial cells of the mucosa and cells associated with lymph nodules; and 4) both SP and SK binding sites expressed by smooth muscle are more stable than SP binding sites expressed by blood vessels, lymph nodules, and mucosal cells.

AB - Quantitative receptor autoradiography was used to localize and quantify the distribution of binding sites for 125I-radiolabeled substance P (SP), substance K (SK) and neuromedin K (NK) in the human GI tract using histologically normal tissue obtained from uninvolved margins of resections for carcinoma. The distribution of SP and SK binding sites is different for each gastrointestinal (GI) segment examined. Specific SP binding sites are expressed by arterioles and venules, myenteric plexus, external circular muscle, external longitudinal muscle, muscularis mucosa, epithelial cells of the mucosa, and the germinal centers of lymph nodules. SK binding sites are distributed in a pattern distinct from SP binding sites and are localized to the external circular muscle, external longitudinal muscle, and the muscularis mucosa. Binding sites for NK were not detected in any part of the human GI tract. These results demonstrate that: 1) surgical specimens from the human GI tract can be effectively processed for quantitative receptor autoradiography; 2) of the three mammalian tachykinins tested, SP and SK, but not NK binding sites are expressed in detectable levels in the human GI tract; 3) whereas SK receptor binding sites are expressed almost exclusively by smooth muscle, SP binding sites are expressed by smooth muscle cells, arterioles, venules, epithelial cells of the mucosa and cells associated with lymph nodules; and 4) both SP and SK binding sites expressed by smooth muscle are more stable than SP binding sites expressed by blood vessels, lymph nodules, and mucosal cells.

KW - Gastrointestinal tract

KW - Receptor

KW - Substance K

KW - Substance P

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