Substance P receptor expression and cellular responses to substance P in prenatal rat spinal cord cells

Paul A St John, Christopher P. Ludwig, Josephine Lai

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Substance Preceptors (SPRs) are expressed by prenatal rat spinal cord neurons and glial cells early in their differentiation, and SPRs may mediate developmental influences in the developing spinal cord. In order to understand better early SPR expression, we quantified SPR mRNA in the rat spinal cord during prenatal development using a cDNA probe for the rat SPR in nuclease protection assays. SPR mRNA was present in the rat spinal cord at E14, the earliest stage examined, and the presence of specific binding sites for radiolabeled SP suggested that SPRs were expressed at the protein level as well. Comparisons of samples from rats at different prenatal ages showed that the relative abundance of SPR mRNA declined by about 75% from E14 through the remainder of prenatal development. Assays of the hydrolysis of phosphatidyl inositol performed on prenatal spinal cord cells in culture revealed that SP caused a small but significant stimulation. These results show that expression of SPRs is an early molecular event in the development of the rat spinal cord in vivo and that SPRs on young spinal cord cells can mediate functional responses at early developmental stages.

Original languageEnglish (US)
Pages (from-to)569-583
Number of pages15
JournalJournal of Receptors and Signal Transduction
Volume17
Issue number4
StatePublished - 1997

Fingerprint

Neurokinin-1 Receptors
Substance P
Rats
Spinal Cord
Messenger RNA
Assays
Nuclease Protection Assays
Phosphatidylinositols
Neuroglia
Neurons
Hydrolysis
Complementary DNA
Cell Culture Techniques
Binding Sites

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Substance P receptor expression and cellular responses to substance P in prenatal rat spinal cord cells. / St John, Paul A; Ludwig, Christopher P.; Lai, Josephine.

In: Journal of Receptors and Signal Transduction, Vol. 17, No. 4, 1997, p. 569-583.

Research output: Contribution to journalArticle

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