Substance P receptors: Localization by light microscopic autoradiography in rat brain using [3H]SP as the radioligand

Patrick W Mantyh, Stephen P. Hunt, John E. Maggio

Research output: Contribution to journalArticle

187 Citations (Scopus)

Abstract

Substance P (SP) is a putative neurotransmitter in both the peripheral and central nervous systems. In the present report we have used a modification of the Young and Kuhar technique to investigate some of the SP receptors binding properties and the distribution of SP receptors in rat brain. Tritiated SP ([3H]SP) adsorbed extensively to glass but this adsorption was greatly reduced by preincubating the slide-mounted tissue sections in a solution containing the cationic polymer polyethylenimine. [3H]SP was found to bind to rat tissue in a saturable fashion with a Bmax of 14.7 fmol/mg tissue wet weight and a Kd of 1.1 nM. The rank order of potencies for displacing [3H]SP binding from rat tissue sections was SP > SP sulphoxide > DiMeC7 > Eledoisin >0. SP(5-11) > SP(COOH) > SP(1-9) amide. Using autoradiography coupled with LKB tritium-sensitive Ultrofilm or the dry emulsion-coated coverslip technique the distribution of [3H]SP binding sites was found to be very dense within the olfactory bulb, amygdalo-hippocampal area and the nucleus of the solitary tract. Heavy concentrations of receptors were observed in the septum, diagonal band of Broca, striatum, subiculum, hypothalamus, locus coeruleus, parabrachial nucleus and lobule 9 and 10 of the cerebellum. Moderate to low concentrations of receptors were observed in the cerebral cortex, globus pallidus, raphe nuclei and the trigeminal nucleus. Very low densities were observed in most aspects of the dorsal thalamus, substantia nigra and cerebellum (other than lobule 9 and 10). Comparisons of the present data with SP peptide levels indicate that in some areas of the brain there is a rough correlation between peptide and receptor levels. However, in other brain areas (olfactory bulb, globus pallidus and substantia nigra) there is little obvious correlation between the two.

Original languageEnglish (US)
Pages (from-to)147-165
Number of pages19
JournalBrain Research
Volume307
Issue number1-2
DOIs
StatePublished - Jul 30 1984
Externally publishedYes

Fingerprint

Neurokinin-1 Receptors
Substance P
Autoradiography
Light
Brain
Globus Pallidus
Olfactory Bulb
Substantia Nigra
Cerebellum
Eledoisin
Diagonal Band of Broca
Trigeminal Nuclei
Polyethyleneimine
Solitary Nucleus
Raphe Nuclei
Peptide Receptors
Locus Coeruleus
Tritium
Peripheral Nervous System
Emulsions

Keywords

  • binding
  • peptide neurotransmitter
  • receptor autoradiography
  • substance P receptors

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

Cite this

Substance P receptors : Localization by light microscopic autoradiography in rat brain using [3H]SP as the radioligand. / Mantyh, Patrick W; Hunt, Stephen P.; Maggio, John E.

In: Brain Research, Vol. 307, No. 1-2, 30.07.1984, p. 147-165.

Research output: Contribution to journalArticle

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