Substitution with selenomethionine can enhance the stability of methionine-rich proteins

Nadine C. Gassner, Walter A. Baase, Andrew Hausrath, Brian W. Matthews

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

The availability of a series of phage T4 lysozymes with up to 14 methionine residues incorporated within the protein has made it possible to systematically compare the effect on protein stability of selenomethionine relative to methionine. Wild-type lysozyme contains two fully buried methionine residues plus three more on the surface. The substitution of these methionine residues with selenomethionine slightly stabilizes the protein. As more and more methionine residues are substituted into the protein, there is a progressive loss of stability. This is, however, increasingly offset in the selenomethionine variants, ultimately resulting in a differential increase in melting temperature of about 7°C. This increase, corresponding to about 0.25 kcal/mol per substitution, is in reasonable agreement with the difference in the solvent transfer free energy between the two amino acids.

Original languageEnglish (US)
Pages (from-to)17-20
Number of pages4
JournalJournal of Molecular Biology
Volume294
Issue number1
DOIs
StatePublished - Nov 19 1999
Externally publishedYes

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Selenomethionine
Methionine
Muramidase
Proteins
Bacteriophage T4
Protein Stability
Energy Transfer
Freezing
Amino Acids
Temperature

Keywords

  • Anomalous scattering
  • MAD phasing
  • Structure determination
  • T4 lysozyme

ASJC Scopus subject areas

  • Virology

Cite this

Substitution with selenomethionine can enhance the stability of methionine-rich proteins. / Gassner, Nadine C.; Baase, Walter A.; Hausrath, Andrew; Matthews, Brian W.

In: Journal of Molecular Biology, Vol. 294, No. 1, 19.11.1999, p. 17-20.

Research output: Contribution to journalArticle

Gassner, Nadine C. ; Baase, Walter A. ; Hausrath, Andrew ; Matthews, Brian W. / Substitution with selenomethionine can enhance the stability of methionine-rich proteins. In: Journal of Molecular Biology. 1999 ; Vol. 294, No. 1. pp. 17-20.
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