Sucrose synthase localization during initiation of seed development and trichome differentiation in cotton ovules

Kurt D Nolte, Donald L. Hendrix, John W. Radin, Karen E. Koch

Research output: Contribution to journalArticle

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Abstract

Sucrose synthase in cotton (Gossypium hirsutum L.) ovules was immunolocalized to clarify the relationship between this enzyme and (a) sucrose import/utilization during initiation of seed development, (b) trichome differentiation, and (c) cell-wall biosynthesis in these rapidly elongating "fibers." Analyses focused on the period immediately before and after trichome initiation (at pollination). Internal tissues most heavily immunolabeled were the developing nucellus, adjacent integument (inner surface), and the vascular region. Little sucrose synthase was associated with the outermost epidermis on the day preceding pollination. However, 1 d later, immunolabel appeared specifically in those epidermal cells at the earliest visible phase of trichome differentiation. The day following pollination, these cells had elongated 3- to 5-fold and showed a further enhancement of sucrose synthase immunolabel. Levels of sucrose synthase mRNA also increased during this period, regardless of whether pollination per se had occurred. Timing of onset for the cell-specific localization of sucrose synthase in young seeds and trichome initials indicates a close association between this enzyme and sucrose import at a cellular level, as well as a potentially integral role in cell-wall biosynthesis.

Original languageEnglish (US)
Pages (from-to)1285-1293
Number of pages9
JournalPlant Physiology
Volume109
Issue number4
StatePublished - Dec 1995
Externally publishedYes

Fingerprint

Trichomes
Ovule
sucrose synthase
seed development
Pollination
ovules
trichomes
Seeds
cotton
pollination
Cell Wall
imports
Sucrose
cell walls
biosynthesis
sucrose
Gossypium
cells
Enzymes
epidermis (plant)

ASJC Scopus subject areas

  • Plant Science

Cite this

Sucrose synthase localization during initiation of seed development and trichome differentiation in cotton ovules. / Nolte, Kurt D; Hendrix, Donald L.; Radin, John W.; Koch, Karen E.

In: Plant Physiology, Vol. 109, No. 4, 12.1995, p. 1285-1293.

Research output: Contribution to journalArticle

Nolte, Kurt D ; Hendrix, Donald L. ; Radin, John W. ; Koch, Karen E. / Sucrose synthase localization during initiation of seed development and trichome differentiation in cotton ovules. In: Plant Physiology. 1995 ; Vol. 109, No. 4. pp. 1285-1293.
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