3H-flunitrazepam binding to bovine retina and the effect of GABA thereon

John W. Regan; William R. Roeske; Henry I. Yamamura

doi:10.1016/0028-3908(80)90195-1

³H-flunitrazepam binding to bovine retina and the effect of GABA thereon

John W. Regan, William R. Roeske, Henry I. Yamamura

Research output: Contribution to journal › Article

17 Scopus citations

Abstract

Bovine retinae were examined for their ability to bind ³H-flunitrazepam. Whole retinal homogenates revealed specific, high affinity, and saturable ³H-flunitrazepam binding (K_d, 1.1 nM; B_max, 19.5 fmoles/mg tissue). In washed membrane preparations, 10 μM GABA caused a dramatic decrease in the K_d (42 percent). The rank order of potency for the inhibition of ³H-flunitrazepam binding by different benzodiazepines was: clonazepam > clobazam ≫ Ro 5-4864. These results are supportive for the presence of benzodiazepine receptors in the bovine retina and indicate a likeness with the previously described benzodiazepine receptors of mammalian brain.

Original language	English (US)
Pages (from-to)	413-414
Number of pages	2
Journal	Neuropharmacology
Volume	19
Issue number	4
DOIs	https://doi.org/10.1016/0028-3908(80)90195-1
State	Published - Apr 1980

ASJC Scopus subject areas

Pharmacology
Cellular and Molecular Neuroscience

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Regan, J. W., Roeske, W. R., & Yamamura, H. I. (1980). ³H-flunitrazepam binding to bovine retina and the effect of GABA thereon. Neuropharmacology, 19(4), 413-414. https://doi.org/10.1016/0028-3908(80)90195-1

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abstract = "Bovine retinae were examined for their ability to bind 3H-flunitrazepam. Whole retinal homogenates revealed specific, high affinity, and saturable 3H-flunitrazepam binding (Kd, 1.1 nM; Bmax, 19.5 fmoles/mg tissue). In washed membrane preparations, 10 μM GABA caused a dramatic decrease in the Kd (42 percent). The rank order of potency for the inhibition of 3H-flunitrazepam binding by different benzodiazepines was: clonazepam > clobazam ≫ Ro 5-4864. These results are supportive for the presence of benzodiazepine receptors in the bovine retina and indicate a likeness with the previously described benzodiazepine receptors of mammalian brain.",

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