3H-flunitrazepam binding to bovine retina and the effect of GABA thereon

John W. Regan, William R. Roeske, Henry I. Yamamura

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

Bovine retinae were examined for their ability to bind 3H-flunitrazepam. Whole retinal homogenates revealed specific, high affinity, and saturable 3H-flunitrazepam binding (Kd, 1.1 nM; Bmax, 19.5 fmoles/mg tissue). In washed membrane preparations, 10 μM GABA caused a dramatic decrease in the Kd (42 percent). The rank order of potency for the inhibition of 3H-flunitrazepam binding by different benzodiazepines was: clonazepam > clobazam ≫ Ro 5-4864. These results are supportive for the presence of benzodiazepine receptors in the bovine retina and indicate a likeness with the previously described benzodiazepine receptors of mammalian brain.

Original languageEnglish (US)
Pages (from-to)413-414
Number of pages2
JournalNeuropharmacology
Volume19
Issue number4
DOIs
StatePublished - Apr 1980

ASJC Scopus subject areas

  • Pharmacology
  • Cellular and Molecular Neuroscience

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