[3H]Pirenzepine identifies putative M1 muscarinic receptors in human stellate ganglia

Mark Watson, William R. Roeske, Peter C. Johnson, Henry I. Yamamura

Research output: Contribution to journalArticle

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Abstract

The specific binding of [3H]pirenzepine ([3H]PZ) and [3H](-) quinuclidinyl benzilate ([3H](-)QNB) was investigated in homogenates of human stellate ganglia. [3H]PZ saturation isotherms yielded a Kd of 14 nM and Bmax of 16.7 fmol/mg protein, while [3H](-)QNB binding curves yielded a Kd of 59 pM and Bmax of 33.0 fmol/mg protein. This represents the greatest proportion of high affinity [3H]PZ labeling, relative to [3H](-)QNB, seen in any peripheral tissue examined thus far.

Original languageEnglish (US)
Pages (from-to)179-182
Number of pages4
JournalBrain Research
Volume290
Issue number1
DOIs
Publication statusPublished - Jan 2 1984
Externally publishedYes

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Keywords

  • ganglia
  • human
  • muscarinic
  • pirenzepine
  • quinuclidinyl benzilate
  • receptors

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

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