[3H]Pirenzepine identifies putative M1 muscarinic receptors in human stellate ganglia

Mark Watson, William R. Roeske, Peter C. Johnson, Henry I. Yamamura

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

The specific binding of [3H]pirenzepine ([3H]PZ) and [3H](-) quinuclidinyl benzilate ([3H](-)QNB) was investigated in homogenates of human stellate ganglia. [3H]PZ saturation isotherms yielded a Kd of 14 nM and Bmax of 16.7 fmol/mg protein, while [3H](-)QNB binding curves yielded a Kd of 59 pM and Bmax of 33.0 fmol/mg protein. This represents the greatest proportion of high affinity [3H]PZ labeling, relative to [3H](-)QNB, seen in any peripheral tissue examined thus far.

Original languageEnglish (US)
Pages (from-to)179-182
Number of pages4
JournalBrain Research
Volume290
Issue number1
DOIs
StatePublished - Jan 2 1984
Externally publishedYes

Fingerprint

Muscarinic M1 Receptors
Stellate Ganglion
Pirenzepine
Quinuclidinyl Benzilate
Proteins

Keywords

  • ganglia
  • human
  • muscarinic
  • pirenzepine
  • quinuclidinyl benzilate
  • receptors

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

Cite this

[3H]Pirenzepine identifies putative M1 muscarinic receptors in human stellate ganglia. / Watson, Mark; Roeske, William R.; Johnson, Peter C.; Yamamura, Henry I.

In: Brain Research, Vol. 290, No. 1, 02.01.1984, p. 179-182.

Research output: Contribution to journalArticle

Watson, Mark ; Roeske, William R. ; Johnson, Peter C. ; Yamamura, Henry I. / [3H]Pirenzepine identifies putative M1 muscarinic receptors in human stellate ganglia. In: Brain Research. 1984 ; Vol. 290, No. 1. pp. 179-182.
@article{68966a6f305e4f0b86b916077e5b2adc,
title = "[3H]Pirenzepine identifies putative M1 muscarinic receptors in human stellate ganglia",
abstract = "The specific binding of [3H]pirenzepine ([3H]PZ) and [3H](-) quinuclidinyl benzilate ([3H](-)QNB) was investigated in homogenates of human stellate ganglia. [3H]PZ saturation isotherms yielded a Kd of 14 nM and Bmax of 16.7 fmol/mg protein, while [3H](-)QNB binding curves yielded a Kd of 59 pM and Bmax of 33.0 fmol/mg protein. This represents the greatest proportion of high affinity [3H]PZ labeling, relative to [3H](-)QNB, seen in any peripheral tissue examined thus far.",
keywords = "ganglia, human, muscarinic, pirenzepine, quinuclidinyl benzilate, receptors",
author = "Mark Watson and Roeske, {William R.} and Johnson, {Peter C.} and Yamamura, {Henry I.}",
year = "1984",
month = "1",
day = "2",
doi = "10.1016/0006-8993(84)90751-0",
language = "English (US)",
volume = "290",
pages = "179--182",
journal = "Brain Research",
issn = "0006-8993",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - [3H]Pirenzepine identifies putative M1 muscarinic receptors in human stellate ganglia

AU - Watson, Mark

AU - Roeske, William R.

AU - Johnson, Peter C.

AU - Yamamura, Henry I.

PY - 1984/1/2

Y1 - 1984/1/2

N2 - The specific binding of [3H]pirenzepine ([3H]PZ) and [3H](-) quinuclidinyl benzilate ([3H](-)QNB) was investigated in homogenates of human stellate ganglia. [3H]PZ saturation isotherms yielded a Kd of 14 nM and Bmax of 16.7 fmol/mg protein, while [3H](-)QNB binding curves yielded a Kd of 59 pM and Bmax of 33.0 fmol/mg protein. This represents the greatest proportion of high affinity [3H]PZ labeling, relative to [3H](-)QNB, seen in any peripheral tissue examined thus far.

AB - The specific binding of [3H]pirenzepine ([3H]PZ) and [3H](-) quinuclidinyl benzilate ([3H](-)QNB) was investigated in homogenates of human stellate ganglia. [3H]PZ saturation isotherms yielded a Kd of 14 nM and Bmax of 16.7 fmol/mg protein, while [3H](-)QNB binding curves yielded a Kd of 59 pM and Bmax of 33.0 fmol/mg protein. This represents the greatest proportion of high affinity [3H]PZ labeling, relative to [3H](-)QNB, seen in any peripheral tissue examined thus far.

KW - ganglia

KW - human

KW - muscarinic

KW - pirenzepine

KW - quinuclidinyl benzilate

KW - receptors

UR - http://www.scopus.com/inward/record.url?scp=0021288810&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021288810&partnerID=8YFLogxK

U2 - 10.1016/0006-8993(84)90751-0

DO - 10.1016/0006-8993(84)90751-0

M3 - Article

C2 - 6546354

AN - SCOPUS:0021288810

VL - 290

SP - 179

EP - 182

JO - Brain Research

JF - Brain Research

SN - 0006-8993

IS - 1

ER -