Superoxide anion (O2-) production by peripheral blood monocytes in Hodgkin's disease and malignant lymphoma

R. Perez-Soler, G. Lopez-Berestein, F. Cabanillas, P. McLaughlin, Evan M Hersh

Research output: Contribution to journalArticle

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Abstract

Superoxide anion (O2-) is the first metabolite of the monocyte oxygen burst pathway, which plays an important role in the monocyte microbicidal function. The capacity of peripheral blood monocytes to produce O2- was studied in 63 patients with Hodgkin's disease (31 with active disease and 32 in complete remission), 15 patients with active malignant lymphoma, and 57 normal control subjects. O2- release was quantified by evaluating superoxide dismutase-inhibitable reduction of cytochrome c after stimulation of monocytes with phorbol myristate acetate. Results were expressed in nanomols O2- per mg protein per hour. O2- production was lower than normal in patients with active Hodgkin's disease (163.3 v 214.5, P < .05). It was normal in patients with Hodgkin's disease in complete remission (216.2 v 214.5, P > .05) and high in patients with malignant lymphomas (317.9 v 214.5, P < .01). Within the group with active Hodgkin's disease, patients in relapse after therapy had a lower O2- production than those previously untreated (99.8 v 181.8, P < .01). Stage of disease was unrelated to the defect. The presence of B symptoms and a decreased delayed type hypersensitivity to recall skin test antigens were associated with normal O2- production. The results obtained suggest that monocyte dysfunction is part of the immune dysregulation associated with active Hodgkin's disease. The O2- determination is a relatively easy test to perform and may be useful in identifying the patients with Hodgkin's disease who have an increased risk of opportunistic infections.

Original languageEnglish (US)
Pages (from-to)641-645
Number of pages5
JournalJournal of Clinical Oncology
Volume3
Issue number5
StatePublished - 1985
Externally publishedYes

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Hodgkin Disease
Superoxides
Monocytes
Lymphoma
Opportunistic Infections
Delayed Hypersensitivity
Tetradecanoylphorbol Acetate
Cytochromes c
Skin Tests
Superoxide Dismutase
Oxygen
Antigens
Recurrence
Proteins

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Superoxide anion (O2-) production by peripheral blood monocytes in Hodgkin's disease and malignant lymphoma. / Perez-Soler, R.; Lopez-Berestein, G.; Cabanillas, F.; McLaughlin, P.; Hersh, Evan M.

In: Journal of Clinical Oncology, Vol. 3, No. 5, 1985, p. 641-645.

Research output: Contribution to journalArticle

Perez-Soler, R, Lopez-Berestein, G, Cabanillas, F, McLaughlin, P & Hersh, EM 1985, 'Superoxide anion (O2-) production by peripheral blood monocytes in Hodgkin's disease and malignant lymphoma', Journal of Clinical Oncology, vol. 3, no. 5, pp. 641-645.
Perez-Soler, R. ; Lopez-Berestein, G. ; Cabanillas, F. ; McLaughlin, P. ; Hersh, Evan M. / Superoxide anion (O2-) production by peripheral blood monocytes in Hodgkin's disease and malignant lymphoma. In: Journal of Clinical Oncology. 1985 ; Vol. 3, No. 5. pp. 641-645.
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