Superresolution three-dimensional images of fluorescence in cells with minimal light exposure

Walter A. Carrington, Ronald M. Lynch, Edwin D.W. Moore, Gerrit Isenberg, Kevin E. Fogarty, Fredric S. Fay

Research output: Contribution to journalArticle

291 Scopus citations

Abstract

Fluorescent probes offer insight into the highly localized and rapid molecular events that underlie cell function. However, methods are required that can efficiently transform the limited signals from such probes into high-resolution images. An algorithm has now been developed that produces highly accurate images of fluorescent probe distribution inside cells with minimal light exposure and a conventional light microscope. This method provides resolution nearly four times greater than that currently available from any fluorescence microscope and was used to study several biological problems.

Original languageEnglish (US)
Pages (from-to)1483-1487
Number of pages5
JournalScience
Volume268
Issue number5216
DOIs
StatePublished - Jan 1 1995

ASJC Scopus subject areas

  • General

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    Carrington, W. A., Lynch, R. M., Moore, E. D. W., Isenberg, G., Fogarty, K. E., & Fay, F. S. (1995). Superresolution three-dimensional images of fluorescence in cells with minimal light exposure. Science, 268(5216), 1483-1487. https://doi.org/10.1126/science.7770772