Superresolution three-dimensional images of fluorescence in cells with minimal light exposure

Walter A. Carrington, Ron Lynch, Edwin D W Moore, Gerrit Isenberg, Kevin E. Fogarty, Fredric S. Fay

Research output: Contribution to journalArticle

287 Citations (Scopus)

Abstract

Fluorescent probes offer insight into the highly localized and rapid molecular events that underlie cell function. However, methods are required that can efficiently transform the limited signals from such probes into high-resolution images. An algorithm has now been developed that produces highly accurate images of fluorescent probe distribution inside cells with minimal light exposure and a conventional light microscope. This method provides resolution nearly four times greater than that currently available from any fluorescence microscope and was used to study several biological problems.

Original languageEnglish (US)
Pages (from-to)1483-1487
Number of pages5
JournalScience
Volume268
Issue number5216
StatePublished - Jun 9 1995

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Three-Dimensional Imaging
Fluorescent Dyes
Fluorescence
Light

ASJC Scopus subject areas

  • General

Cite this

Carrington, W. A., Lynch, R., Moore, E. D. W., Isenberg, G., Fogarty, K. E., & Fay, F. S. (1995). Superresolution three-dimensional images of fluorescence in cells with minimal light exposure. Science, 268(5216), 1483-1487.

Superresolution three-dimensional images of fluorescence in cells with minimal light exposure. / Carrington, Walter A.; Lynch, Ron; Moore, Edwin D W; Isenberg, Gerrit; Fogarty, Kevin E.; Fay, Fredric S.

In: Science, Vol. 268, No. 5216, 09.06.1995, p. 1483-1487.

Research output: Contribution to journalArticle

Carrington, WA, Lynch, R, Moore, EDW, Isenberg, G, Fogarty, KE & Fay, FS 1995, 'Superresolution three-dimensional images of fluorescence in cells with minimal light exposure', Science, vol. 268, no. 5216, pp. 1483-1487.
Carrington WA, Lynch R, Moore EDW, Isenberg G, Fogarty KE, Fay FS. Superresolution three-dimensional images of fluorescence in cells with minimal light exposure. Science. 1995 Jun 9;268(5216):1483-1487.
Carrington, Walter A. ; Lynch, Ron ; Moore, Edwin D W ; Isenberg, Gerrit ; Fogarty, Kevin E. ; Fay, Fredric S. / Superresolution three-dimensional images of fluorescence in cells with minimal light exposure. In: Science. 1995 ; Vol. 268, No. 5216. pp. 1483-1487.
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