Supporting material affects the growth and development of in vitro sweet potato plantlets cultured photoautotrophically

F. Afreen-Zobayed, S. M A Zobayed, Chieri Kubota, T. Kozai, O. Hasegawa

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

A comparative study was conducted to optimize the vegetative growth of sweet potato (Ipomoea batatas L. (Lam), cv. Beniazuma) plantlets cultured in vitro in five different types of supporting materials: agar matrix (a seaweed derivative; Kanto Chemical Co. Inc., Tokyo), gellan gum (a Pseudomonas derivative; Kanto Chemical Co. Inc., Tokyo), vermiculite (a kind of hydrous silicates), a mixture of vermiculite and cellulose fiber (Florialite; Nisshinbo Industries, Inc., Tokyo) and cellulose plug (Sorbarod; Baumgartner Rapiers SA., Switzerland). Single nodal cuttings were cultured photoautotrophically (without any sugar in the medium and with enriched CO2 and high photosynthetic photon flux) for 21 d on MS basal medium. Plantlets exhibited the greatest growth when Florialite was used as supporting material. The leaf and root fresh and dry mass were 2.4X and 2.9X, and 2.2X and 2.8X greater, respectively, than those of the plantlets grown in the agar matrix (control). Plantlets cultured in Sorbarod supporting material exhibited the second greatest fresh and dry mass of leaves and roots followed by vermiculite and gellan gum supporting material. The most interesting feature was the development of a large number of fine lateral roots from the main adventitious root in the Florialite treatment. Among the treatments, the highest net photosynthetic rate was evident in the Florialite grown plantlets. The percent porosity of the supporting materials was highest in Sorbarod followed by Florialite and vermiculite. Plantlets transplanted from the Florialite supporting material exhibited the highest acclimatization percentage followed by that of the Sorbarod treatment.

Original languageEnglish (US)
Pages (from-to)470-474
Number of pages5
JournalIn Vitro Cellular and Developmental Biology - Plant
Volume35
Issue number6
StatePublished - Nov 1999
Externally publishedYes

Fingerprint

Ipomoea batatas
sweet potatoes
Growth and Development
Tokyo
plantlets
growth and development
vermiculite
Cellulose
Agar
gellan gum
Derivatives
Seaweed
Silicates
Acclimatization
Porosity
Growth
Pseudomonas
agar
Switzerland
Photons

Keywords

  • Agar
  • Cellulose fiber
  • CO enrichment
  • Root
  • Vermiculite

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology
  • Developmental Biology
  • Embryology

Cite this

Supporting material affects the growth and development of in vitro sweet potato plantlets cultured photoautotrophically. / Afreen-Zobayed, F.; Zobayed, S. M A; Kubota, Chieri; Kozai, T.; Hasegawa, O.

In: In Vitro Cellular and Developmental Biology - Plant, Vol. 35, No. 6, 11.1999, p. 470-474.

Research output: Contribution to journalArticle

@article{a667c9d1f41c473e9d27c0ad111967a1,
title = "Supporting material affects the growth and development of in vitro sweet potato plantlets cultured photoautotrophically",
abstract = "A comparative study was conducted to optimize the vegetative growth of sweet potato (Ipomoea batatas L. (Lam), cv. Beniazuma) plantlets cultured in vitro in five different types of supporting materials: agar matrix (a seaweed derivative; Kanto Chemical Co. Inc., Tokyo), gellan gum (a Pseudomonas derivative; Kanto Chemical Co. Inc., Tokyo), vermiculite (a kind of hydrous silicates), a mixture of vermiculite and cellulose fiber (Florialite; Nisshinbo Industries, Inc., Tokyo) and cellulose plug (Sorbarod; Baumgartner Rapiers SA., Switzerland). Single nodal cuttings were cultured photoautotrophically (without any sugar in the medium and with enriched CO2 and high photosynthetic photon flux) for 21 d on MS basal medium. Plantlets exhibited the greatest growth when Florialite was used as supporting material. The leaf and root fresh and dry mass were 2.4X and 2.9X, and 2.2X and 2.8X greater, respectively, than those of the plantlets grown in the agar matrix (control). Plantlets cultured in Sorbarod supporting material exhibited the second greatest fresh and dry mass of leaves and roots followed by vermiculite and gellan gum supporting material. The most interesting feature was the development of a large number of fine lateral roots from the main adventitious root in the Florialite treatment. Among the treatments, the highest net photosynthetic rate was evident in the Florialite grown plantlets. The percent porosity of the supporting materials was highest in Sorbarod followed by Florialite and vermiculite. Plantlets transplanted from the Florialite supporting material exhibited the highest acclimatization percentage followed by that of the Sorbarod treatment.",
keywords = "Agar, Cellulose fiber, CO enrichment, Root, Vermiculite",
author = "F. Afreen-Zobayed and Zobayed, {S. M A} and Chieri Kubota and T. Kozai and O. Hasegawa",
year = "1999",
month = "11",
language = "English (US)",
volume = "35",
pages = "470--474",
journal = "In Vitro Cellular and Developmental Biology - Plant",
issn = "1054-5476",
publisher = "Springer New York",
number = "6",

}

TY - JOUR

T1 - Supporting material affects the growth and development of in vitro sweet potato plantlets cultured photoautotrophically

AU - Afreen-Zobayed, F.

AU - Zobayed, S. M A

AU - Kubota, Chieri

AU - Kozai, T.

AU - Hasegawa, O.

PY - 1999/11

Y1 - 1999/11

N2 - A comparative study was conducted to optimize the vegetative growth of sweet potato (Ipomoea batatas L. (Lam), cv. Beniazuma) plantlets cultured in vitro in five different types of supporting materials: agar matrix (a seaweed derivative; Kanto Chemical Co. Inc., Tokyo), gellan gum (a Pseudomonas derivative; Kanto Chemical Co. Inc., Tokyo), vermiculite (a kind of hydrous silicates), a mixture of vermiculite and cellulose fiber (Florialite; Nisshinbo Industries, Inc., Tokyo) and cellulose plug (Sorbarod; Baumgartner Rapiers SA., Switzerland). Single nodal cuttings were cultured photoautotrophically (without any sugar in the medium and with enriched CO2 and high photosynthetic photon flux) for 21 d on MS basal medium. Plantlets exhibited the greatest growth when Florialite was used as supporting material. The leaf and root fresh and dry mass were 2.4X and 2.9X, and 2.2X and 2.8X greater, respectively, than those of the plantlets grown in the agar matrix (control). Plantlets cultured in Sorbarod supporting material exhibited the second greatest fresh and dry mass of leaves and roots followed by vermiculite and gellan gum supporting material. The most interesting feature was the development of a large number of fine lateral roots from the main adventitious root in the Florialite treatment. Among the treatments, the highest net photosynthetic rate was evident in the Florialite grown plantlets. The percent porosity of the supporting materials was highest in Sorbarod followed by Florialite and vermiculite. Plantlets transplanted from the Florialite supporting material exhibited the highest acclimatization percentage followed by that of the Sorbarod treatment.

AB - A comparative study was conducted to optimize the vegetative growth of sweet potato (Ipomoea batatas L. (Lam), cv. Beniazuma) plantlets cultured in vitro in five different types of supporting materials: agar matrix (a seaweed derivative; Kanto Chemical Co. Inc., Tokyo), gellan gum (a Pseudomonas derivative; Kanto Chemical Co. Inc., Tokyo), vermiculite (a kind of hydrous silicates), a mixture of vermiculite and cellulose fiber (Florialite; Nisshinbo Industries, Inc., Tokyo) and cellulose plug (Sorbarod; Baumgartner Rapiers SA., Switzerland). Single nodal cuttings were cultured photoautotrophically (without any sugar in the medium and with enriched CO2 and high photosynthetic photon flux) for 21 d on MS basal medium. Plantlets exhibited the greatest growth when Florialite was used as supporting material. The leaf and root fresh and dry mass were 2.4X and 2.9X, and 2.2X and 2.8X greater, respectively, than those of the plantlets grown in the agar matrix (control). Plantlets cultured in Sorbarod supporting material exhibited the second greatest fresh and dry mass of leaves and roots followed by vermiculite and gellan gum supporting material. The most interesting feature was the development of a large number of fine lateral roots from the main adventitious root in the Florialite treatment. Among the treatments, the highest net photosynthetic rate was evident in the Florialite grown plantlets. The percent porosity of the supporting materials was highest in Sorbarod followed by Florialite and vermiculite. Plantlets transplanted from the Florialite supporting material exhibited the highest acclimatization percentage followed by that of the Sorbarod treatment.

KW - Agar

KW - Cellulose fiber

KW - CO enrichment

KW - Root

KW - Vermiculite

UR - http://www.scopus.com/inward/record.url?scp=0033371556&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033371556&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:0033371556

VL - 35

SP - 470

EP - 474

JO - In Vitro Cellular and Developmental Biology - Plant

JF - In Vitro Cellular and Developmental Biology - Plant

SN - 1054-5476

IS - 6

ER -