Suppression of cytochrome P450 reductase expression promotes astrocytosis in subventricular zone of adult mice

Yunyi Yao, Senyan Liu, Yue Wang, Weiping Yuan, Xinxin Ding, Tao Cheng, Qin Shen, Jun Gu

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

The aim of this study was to determine the role of NADPH-cytochrome P450 reductase (CPR) and CPR-dependent enzymes in neural stem cell (NSC) genesis in the brain. A mouse model with globally suppressed Cpr gene expression (Cpr-low mouse) was studied for this purpose. Cpr-low and wild-type (WT) mice were compared immunohistochemically for the expression of markers of cell proliferation (Ki67), immature neurons (doublecortin, DCX), oligodendrocytes (oligodendrocyte transcription factor 2, OLIG2), and astrocytes (glial fibrillary acidic protein, GFAP) in the SVZ, and for the in vitro capability of their SVZ cells to form neurospheres and differentiate into astrocytes. We found that the abundance of SVZ cells that are positive for Ki67 or GFAP expression, but not the abundance of SVZ cells that are positive for DCX and OLIG2 expression, was significantly increased in Cpr-low mice, at various ages, compared with WT mice. Furthermore, extents of astrocyte differentiation and growth, but not neurosphere formation, from SVZ cells of the Cpr-low mice were significantly increased, compared with WT mice. These results suggest that CPR and CPR-dependent enzymes play a role in suppressing astrocytosis in the SVZ of adult mice.

Original languageEnglish (US)
Pages (from-to)84-89
Number of pages6
JournalNeuroscience Letters
Volume548
DOIs
StatePublished - Aug 26 2013
Externally publishedYes

Keywords

  • Astrocytosis
  • Cytochrome P450 reductase
  • Mice
  • Neural stem cells
  • Subventricular zone

ASJC Scopus subject areas

  • Neuroscience(all)

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