Suppression of the cup-5 mucolipidosis type IV-related lysosomal dysfunction by the inactivation of an ABC transporter in C. elegans

Lara Schaheen, Greg Patton, Hanna F Fares

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Mutations in MCOLN1, which encodes the protein mucolipin 1, result in the lysosomal storage disease mucolipidosis Type IV. Studies on human mucolipin 1 and on CUP-5, the Caenorhabditis elegans ortholog of mucolipin 1, have shown that these proteins are required for lysosome biogenesis/function. Loss of CUP-5 results in a defect in lysosomal degradation, leading to embryonic lethality. We have identified a mutation in the ABC transporter MRP-4 that rescues the degradation defect and the corresponding lethality, owing to the absence of CUP-5. MRP-4 localizes to endocytic compartments and its levels are elevated in the absence of CUP-5. These results indicate that the lysosomal degradation defect is exacerbated in some cells because of the accumulation of MRP-4 in lysosomes rather than the loss of CUP-5 per se. We also show that under some conditions, loss of MRP-4 rescues the embryonic lethality caused by the loss of the cathepsin L protease, indicating that the accumulation of ABC transporters may be a more general mechanism whereby an initial lysosomal dysfunction is more severely compromised.

Original languageEnglish (US)
Pages (from-to)3939-3948
Number of pages10
JournalDevelopment
Volume133
Issue number19
DOIs
StatePublished - Oct 2006

Fingerprint

Mucolipidoses
ATP-Binding Cassette Transporters
Lysosomes
Lysosomal Storage Diseases
Cathepsin L
Mutation
Caenorhabditis elegans
Proteins
Peptide Hydrolases

Keywords

  • ABC transporter
  • C. elegans
  • CUP-5
  • MRP-4
  • Mucolipidosis Type IV

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Cite this

Suppression of the cup-5 mucolipidosis type IV-related lysosomal dysfunction by the inactivation of an ABC transporter in C. elegans. / Schaheen, Lara; Patton, Greg; Fares, Hanna F.

In: Development, Vol. 133, No. 19, 10.2006, p. 3939-3948.

Research output: Contribution to journalArticle

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