Suppression or differentiation of solvent resonance by a combination of DEFT with a two-dimensional sequence

Lung Fa Kao, Victor J Hruby

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

A new pulse sequence is described which combines the driven equilibrium Fourier transform (DEFT) and 2D pulse scheme, and allows simultaneous suppression of the solvent peak (H2O/D2O) in 1D and 2D experiments without perturbing the nearby solute signals. To overcome the sensitivity problem, and to allow for 2D experiments in a reasonably short time, a steady-state condition was set up by adjusting τ1 (pulse delay) and D (recycle delay) to be 2-4 times the longest spin-lattice relaxation time T1 (peptide) such that Mz (solvent) was nulled prior to the 2D pulse sequence. Longitudinal and transverse relaxation rates of the solvent were analyzed in the steady state, and a direct calculation of T1 (solvent) was performed using an equation derived in the present work. This novel pulse sequence is also extended to 2D NOE experiments with effective solvent suppression. The method is demonstrated in a variety of 1D and 2D experiments on the cyclic delta opioid receptor selective peptide {A figure is presented}.

Original languageEnglish (US)
Pages (from-to)394-407
Number of pages14
JournalJournal of Magnetic Resonance (1969)
Volume70
Issue number3
DOIs
Publication statusPublished - 1986

    Fingerprint

Cite this