Suppressor analyses of temperature-sensitive cbp1 strains of Saccharomyces cerevisiae: The product of the nuclear gene SOC1 affects mitochondrial cytochrome b mRNA post-transcriptionally

R. R. Staples, Carol L Dieckmann

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The induction of mitochondrial function is dependent upon both nuclearly encoded and mitochondrially encoded gene products. To understand nuclear- mitochondrial interactions, we must first understand gene-specific interactions. The accumulation of mitochondrial cytochrome b (COB) RNA is dependent upon Cbp1p, encoded by the nuclear gene CBP1. Thus, respiration is dependent upon Cbp1p. In this study, suppressors of temperature-sensitive cbp1 (cbp1(ts)) strains were selected for restoration of respiratory capability at the restrictive temperature (Ts+). One nuclearly encoded suppressor, extragenic to CBP1, is recessive with respect to the wild-type suppressor allele and is unlinked to other known genetic loci whose gene products are necessary for expression of COB mRNA. The suppressor, called soc1 for Suppressor of cbp1, suppresses several other cbp1(ts) alleles but does not operate via a bypass mechanism. Molecular analyses indicate that soc1 allows the steady-state level of COB mRNA to increase at high temperature but has little or no effect on the levels of COB pre-mRNA. These data have led us to propose that the product of the nuclear gene SOC1 is required for normal turnover of COB mRNA.

Original languageEnglish (US)
Pages (from-to)565-575
Number of pages11
JournalGenetics
Volume138
Issue number3
StatePublished - 1994
Externally publishedYes

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Cytochromes b
Saccharomyces cerevisiae
Messenger RNA
Temperature
Genes
Alleles
Genetic Loci
RNA Precursors
Respiration
RNA

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

Cite this

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title = "Suppressor analyses of temperature-sensitive cbp1 strains of Saccharomyces cerevisiae: The product of the nuclear gene SOC1 affects mitochondrial cytochrome b mRNA post-transcriptionally",
abstract = "The induction of mitochondrial function is dependent upon both nuclearly encoded and mitochondrially encoded gene products. To understand nuclear- mitochondrial interactions, we must first understand gene-specific interactions. The accumulation of mitochondrial cytochrome b (COB) RNA is dependent upon Cbp1p, encoded by the nuclear gene CBP1. Thus, respiration is dependent upon Cbp1p. In this study, suppressors of temperature-sensitive cbp1 (cbp1(ts)) strains were selected for restoration of respiratory capability at the restrictive temperature (Ts+). One nuclearly encoded suppressor, extragenic to CBP1, is recessive with respect to the wild-type suppressor allele and is unlinked to other known genetic loci whose gene products are necessary for expression of COB mRNA. The suppressor, called soc1 for Suppressor of cbp1, suppresses several other cbp1(ts) alleles but does not operate via a bypass mechanism. Molecular analyses indicate that soc1 allows the steady-state level of COB mRNA to increase at high temperature but has little or no effect on the levels of COB pre-mRNA. These data have led us to propose that the product of the nuclear gene SOC1 is required for normal turnover of COB mRNA.",
author = "Staples, {R. R.} and Dieckmann, {Carol L}",
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T1 - Suppressor analyses of temperature-sensitive cbp1 strains of Saccharomyces cerevisiae

T2 - The product of the nuclear gene SOC1 affects mitochondrial cytochrome b mRNA post-transcriptionally

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AU - Dieckmann, Carol L

PY - 1994

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N2 - The induction of mitochondrial function is dependent upon both nuclearly encoded and mitochondrially encoded gene products. To understand nuclear- mitochondrial interactions, we must first understand gene-specific interactions. The accumulation of mitochondrial cytochrome b (COB) RNA is dependent upon Cbp1p, encoded by the nuclear gene CBP1. Thus, respiration is dependent upon Cbp1p. In this study, suppressors of temperature-sensitive cbp1 (cbp1(ts)) strains were selected for restoration of respiratory capability at the restrictive temperature (Ts+). One nuclearly encoded suppressor, extragenic to CBP1, is recessive with respect to the wild-type suppressor allele and is unlinked to other known genetic loci whose gene products are necessary for expression of COB mRNA. The suppressor, called soc1 for Suppressor of cbp1, suppresses several other cbp1(ts) alleles but does not operate via a bypass mechanism. Molecular analyses indicate that soc1 allows the steady-state level of COB mRNA to increase at high temperature but has little or no effect on the levels of COB pre-mRNA. These data have led us to propose that the product of the nuclear gene SOC1 is required for normal turnover of COB mRNA.

AB - The induction of mitochondrial function is dependent upon both nuclearly encoded and mitochondrially encoded gene products. To understand nuclear- mitochondrial interactions, we must first understand gene-specific interactions. The accumulation of mitochondrial cytochrome b (COB) RNA is dependent upon Cbp1p, encoded by the nuclear gene CBP1. Thus, respiration is dependent upon Cbp1p. In this study, suppressors of temperature-sensitive cbp1 (cbp1(ts)) strains were selected for restoration of respiratory capability at the restrictive temperature (Ts+). One nuclearly encoded suppressor, extragenic to CBP1, is recessive with respect to the wild-type suppressor allele and is unlinked to other known genetic loci whose gene products are necessary for expression of COB mRNA. The suppressor, called soc1 for Suppressor of cbp1, suppresses several other cbp1(ts) alleles but does not operate via a bypass mechanism. Molecular analyses indicate that soc1 allows the steady-state level of COB mRNA to increase at high temperature but has little or no effect on the levels of COB pre-mRNA. These data have led us to propose that the product of the nuclear gene SOC1 is required for normal turnover of COB mRNA.

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