Suppressor cell activity among the peripheral blood leukocytes of selected homosexual subjects

Evan M Hersh, P. W A Mansell, J. M. Reuben, J. Frank, A. Rios, R. LaPushin, G. Newell

Research output: Contribution to journalArticle

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Abstract

Suppressor cell activity was investigated in the peripheral blood leukocytes of 21 homosexual subjects with a presumed prodrome of the opportunistic infection, Kaposi's sarcoma syndrome. Patient lymphocytes, either untreated or irradiated with 4000 rads, were cultured alone or were cocultured with normal lymphocytes. The cultures were stimulated with phytohemagglutinin, concanavalin A, and pokeweed mitogen. The proliferative responses of these cultures were compared to each other and were related to concurrently studied delayed hypersensitivity skin test responses to dermatophytin, candida, varidase, mumps, and purified protein derivative of tuberculoprotein; to the proportion of erythrocyte rosette-forming cells and OKY 3-, OKT 4-, and OKT 8-positive cells; and the the OKT 4:OKT 8 ratio in the peripheral blood leukocytes. Patient blastogenic responses were significantly lower than controls for phytohemagglutinin (29.9 versus 75.3 x 103 cpm/culture), for concanavalin A (6.9 versus 28.7 x 103 cpm/culture), and for pokeweed mitogen (7.1 versus 22.4 x 103 cpm/culture). In 12 of 21 patients, significant suppressor cell activity was detected for all three mitogens. For example, for these 12, the normal controls showed 82.0 x 103 cpm when cultured alone but only 0.9 x 103 cpm when cocultured with the patient's cells. Irradiation reversed the suppressor cell activity in none of 12 of the subjects. Suppressor cell activity was seen with as few as 0.125 x 105 patient lymphocytes added to 1.5 x 105 normal lymphocytes. The percentage of suppression of the normal blastogenic responses correlated significantly with the patient blastogenic responses, the percentage of erythrocyte rosette-forming cells, and the helper:suppressor ratio, but not with the percentage of suppressor cells. These data indicate that suppressor cell activity can be found in homosexuals with the prodrome of the opportunistic infection, Kaposi's sarcoma syndrome, and may be responsible for their in vivo immune deficiency. It also suggests that therapeutic maneuvers, which would restore the helper:suppressor ratio towards normal, might benefit the patients clinically.

Original languageEnglish (US)
Pages (from-to)1905-1909
Number of pages5
JournalCancer Research
Volume43
Issue number4
StatePublished - 1983
Externally publishedYes

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Leukocytes
Lymphocytes
Pokeweed Mitogens
Kaposi's Sarcoma
Opportunistic Infections
Phytohemagglutinins
Concanavalin A
Streptodornase and Streptokinase
Erythrocytes
Sexual Minorities
Mumps
Delayed Hypersensitivity
Helper-Inducer T-Lymphocytes
Skin Tests
Mitogens
Candida
Proteins

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Hersh, E. M., Mansell, P. W. A., Reuben, J. M., Frank, J., Rios, A., LaPushin, R., & Newell, G. (1983). Suppressor cell activity among the peripheral blood leukocytes of selected homosexual subjects. Cancer Research, 43(4), 1905-1909.

Suppressor cell activity among the peripheral blood leukocytes of selected homosexual subjects. / Hersh, Evan M; Mansell, P. W A; Reuben, J. M.; Frank, J.; Rios, A.; LaPushin, R.; Newell, G.

In: Cancer Research, Vol. 43, No. 4, 1983, p. 1905-1909.

Research output: Contribution to journalArticle

Hersh, EM, Mansell, PWA, Reuben, JM, Frank, J, Rios, A, LaPushin, R & Newell, G 1983, 'Suppressor cell activity among the peripheral blood leukocytes of selected homosexual subjects', Cancer Research, vol. 43, no. 4, pp. 1905-1909.
Hersh EM, Mansell PWA, Reuben JM, Frank J, Rios A, LaPushin R et al. Suppressor cell activity among the peripheral blood leukocytes of selected homosexual subjects. Cancer Research. 1983;43(4):1905-1909.
Hersh, Evan M ; Mansell, P. W A ; Reuben, J. M. ; Frank, J. ; Rios, A. ; LaPushin, R. ; Newell, G. / Suppressor cell activity among the peripheral blood leukocytes of selected homosexual subjects. In: Cancer Research. 1983 ; Vol. 43, No. 4. pp. 1905-1909.
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