Surface analysis of early and late MOPC-315-EL tumor cells

David T. Harris, Jennifer Bailey, Jon C. Lewis, William H. Grover, Marguerite Panetti, Donald L. Evans, Mary J. Ruebush, Leslie K. Paulus, Donald Burgess, Arthur H. Hale

Research output: Contribution to journalArticle

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Abstract

The surface properties of a tumor line which undergoes a cyclic change in reactivity with cytotoxic T lymphocytes (CTLs) have been analyzed. The findings indicate that MOPC-315-EL when transplanted into the peritoneum of a BALB/c mouse for 4-5 days (early MOPC315-EL) becomes sensitive to lysis by anti-H-2d CTL. After 9 to 10 days of growth in the peritoneal cavity these tumor cells (late MOPC-315-EL) become unreactive to CTLs. The amount of H-2 antigens on the surface of the cells is the same on the early and late tumor cells. By scanning electron microscopy (SEM) we have shown that the early population is a mixture of two cell types which are characterized by surface villi or surface blebs; the late cells are essentially one population which are characterized by surface villi. The net charge on the surface of the cell was evaluated by two methods: (i) rate of cell migration across an electric potential and (ii) binding by cationic ferritin to the glycocalyx. Both techniques indicated that the early cells have a higher net negative charge. The implication of this work is that surface charge may be involved in T cell-target cell interactions.

Original languageEnglish (US)
Pages (from-to)119-130
Number of pages12
JournalCellular Immunology
Volume64
Issue number1
DOIs
StatePublished - 1981
Externally publishedYes

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Cytotoxic T-Lymphocytes
Neoplasms
H-2 Antigens
Glycocalyx
Surface Properties
Peritoneum
Peritoneal Cavity
Blister
Ferritins
Cell Communication
Electron Scanning Microscopy
Population
Cell Movement
T-Lymphocytes
Growth

ASJC Scopus subject areas

  • Cell Biology
  • Immunology

Cite this

Harris, D. T., Bailey, J., Lewis, J. C., Grover, W. H., Panetti, M., Evans, D. L., ... Hale, A. H. (1981). Surface analysis of early and late MOPC-315-EL tumor cells. Cellular Immunology, 64(1), 119-130. https://doi.org/10.1016/0008-8749(81)90463-9

Surface analysis of early and late MOPC-315-EL tumor cells. / Harris, David T.; Bailey, Jennifer; Lewis, Jon C.; Grover, William H.; Panetti, Marguerite; Evans, Donald L.; Ruebush, Mary J.; Paulus, Leslie K.; Burgess, Donald; Hale, Arthur H.

In: Cellular Immunology, Vol. 64, No. 1, 1981, p. 119-130.

Research output: Contribution to journalArticle

Harris, DT, Bailey, J, Lewis, JC, Grover, WH, Panetti, M, Evans, DL, Ruebush, MJ, Paulus, LK, Burgess, D & Hale, AH 1981, 'Surface analysis of early and late MOPC-315-EL tumor cells', Cellular Immunology, vol. 64, no. 1, pp. 119-130. https://doi.org/10.1016/0008-8749(81)90463-9
Harris, David T. ; Bailey, Jennifer ; Lewis, Jon C. ; Grover, William H. ; Panetti, Marguerite ; Evans, Donald L. ; Ruebush, Mary J. ; Paulus, Leslie K. ; Burgess, Donald ; Hale, Arthur H. / Surface analysis of early and late MOPC-315-EL tumor cells. In: Cellular Immunology. 1981 ; Vol. 64, No. 1. pp. 119-130.
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