Sustained Activation of Akt Elicits Mitochondrial Dysfunction to Block Plasmodium falciparum Infection in the Mosquito Host

Shirley Luckhart, Cecilia Giulivi, Anna L. Drexler, Yevgeniya Antonova-Koch, Danielle Sakaguchi, Eleonora Napoli, Sarah Wong, Mark S. Price, Richard Eigenheer, Brett S. Phinney, Nazzy Pakpour, Jose E. Pietri, Kong Cheung, Martha Georgis, Michael A Riehle

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

The overexpression of activated, myristoylated Akt in the midgut of female transgenic Anopheles stephensi results in resistance to infection with the human malaria parasite Plasmodium falciparum but also decreased lifespan. In the present study, the understanding of mitochondria-dependent midgut homeostasis has been expanded to explain this apparent paradox in an insect of major medical importance. Given that Akt signaling is essential for cell growth and survival, we hypothesized that sustained Akt activation in the mosquito midgut would alter the balance of critical pathways that control mitochondrial dynamics to enhance parasite killing at some cost to survivorship. Toxic reactive oxygen and nitrogen species (RNOS) rise to high levels in the midgut after blood feeding, due to a combination of high NO production and a decline in FOXO-dependent antioxidants. Despite an apparent increase in mitochondrial biogenesis in young females (3 d), energy deficiencies were apparent as decreased oxidative phosphorylation and increased [AMP]/[ATP] ratios. In addition, mitochondrial mass was lower and accompanied by the presence of stalled autophagosomes in the posterior midgut, a critical site for blood digestion and stem cell-mediated epithelial maintenance and repair, and by functional degradation of the epithelial barrier. By 18 d, the age at which An. stephensi would transmit P. falciparum to human hosts, mitochondrial dysfunction coupled to Akt-mediated repression of autophagy/mitophagy was more evident and midgut epithelial structure was markedly compromised. Inhibition of RNOS by co-feeding of the nitric-oxide synthase inhibitor L-NAME at infection abrogated Akt-dependent killing of P. falciparum that begins within 18 h of infection in 3-5 d old mosquitoes. Hence, Akt-induced changes in mitochondrial dynamics perturb midgut homeostasis to enhance parasite resistance and decrease mosquito infective lifespan. Further, quality control of mitochondrial function in the midgut is necessary for the maintenance of midgut health as reflected in energy homeostasis and tissue repair and renewal.

Original languageEnglish (US)
Article numbere1003180
JournalPLoS Pathogens
Volume9
Issue number2
DOIs
StatePublished - Feb 2013

Fingerprint

Plasmodium falciparum
Culicidae
Mitochondrial Dynamics
Malaria
Reactive Nitrogen Species
Parasites
Homeostasis
Reactive Oxygen Species
Mitochondrial Degradation
Infection
Anopheles
Critical Pathways
Falciparum Malaria
Poisons
Oxidative Phosphorylation
NG-Nitroarginine Methyl Ester
Autophagy
Organelle Biogenesis
Adenosine Monophosphate
Nitric Oxide Synthase

ASJC Scopus subject areas

  • Microbiology
  • Parasitology
  • Virology
  • Immunology
  • Genetics
  • Molecular Biology

Cite this

Sustained Activation of Akt Elicits Mitochondrial Dysfunction to Block Plasmodium falciparum Infection in the Mosquito Host. / Luckhart, Shirley; Giulivi, Cecilia; Drexler, Anna L.; Antonova-Koch, Yevgeniya; Sakaguchi, Danielle; Napoli, Eleonora; Wong, Sarah; Price, Mark S.; Eigenheer, Richard; Phinney, Brett S.; Pakpour, Nazzy; Pietri, Jose E.; Cheung, Kong; Georgis, Martha; Riehle, Michael A.

In: PLoS Pathogens, Vol. 9, No. 2, e1003180, 02.2013.

Research output: Contribution to journalArticle

Luckhart, S, Giulivi, C, Drexler, AL, Antonova-Koch, Y, Sakaguchi, D, Napoli, E, Wong, S, Price, MS, Eigenheer, R, Phinney, BS, Pakpour, N, Pietri, JE, Cheung, K, Georgis, M & Riehle, MA 2013, 'Sustained Activation of Akt Elicits Mitochondrial Dysfunction to Block Plasmodium falciparum Infection in the Mosquito Host', PLoS Pathogens, vol. 9, no. 2, e1003180. https://doi.org/10.1371/journal.ppat.1003180
Luckhart, Shirley ; Giulivi, Cecilia ; Drexler, Anna L. ; Antonova-Koch, Yevgeniya ; Sakaguchi, Danielle ; Napoli, Eleonora ; Wong, Sarah ; Price, Mark S. ; Eigenheer, Richard ; Phinney, Brett S. ; Pakpour, Nazzy ; Pietri, Jose E. ; Cheung, Kong ; Georgis, Martha ; Riehle, Michael A. / Sustained Activation of Akt Elicits Mitochondrial Dysfunction to Block Plasmodium falciparum Infection in the Mosquito Host. In: PLoS Pathogens. 2013 ; Vol. 9, No. 2.
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abstract = "The overexpression of activated, myristoylated Akt in the midgut of female transgenic Anopheles stephensi results in resistance to infection with the human malaria parasite Plasmodium falciparum but also decreased lifespan. In the present study, the understanding of mitochondria-dependent midgut homeostasis has been expanded to explain this apparent paradox in an insect of major medical importance. Given that Akt signaling is essential for cell growth and survival, we hypothesized that sustained Akt activation in the mosquito midgut would alter the balance of critical pathways that control mitochondrial dynamics to enhance parasite killing at some cost to survivorship. Toxic reactive oxygen and nitrogen species (RNOS) rise to high levels in the midgut after blood feeding, due to a combination of high NO production and a decline in FOXO-dependent antioxidants. Despite an apparent increase in mitochondrial biogenesis in young females (3 d), energy deficiencies were apparent as decreased oxidative phosphorylation and increased [AMP]/[ATP] ratios. In addition, mitochondrial mass was lower and accompanied by the presence of stalled autophagosomes in the posterior midgut, a critical site for blood digestion and stem cell-mediated epithelial maintenance and repair, and by functional degradation of the epithelial barrier. By 18 d, the age at which An. stephensi would transmit P. falciparum to human hosts, mitochondrial dysfunction coupled to Akt-mediated repression of autophagy/mitophagy was more evident and midgut epithelial structure was markedly compromised. Inhibition of RNOS by co-feeding of the nitric-oxide synthase inhibitor L-NAME at infection abrogated Akt-dependent killing of P. falciparum that begins within 18 h of infection in 3-5 d old mosquitoes. Hence, Akt-induced changes in mitochondrial dynamics perturb midgut homeostasis to enhance parasite resistance and decrease mosquito infective lifespan. Further, quality control of mitochondrial function in the midgut is necessary for the maintenance of midgut health as reflected in energy homeostasis and tissue repair and renewal.",
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