Symbiodinium (Pyrrhophyta) genome sizes (DNA content) are smallest among dinoflagellates

Todd C. LaJeunesse, Georgina Lambert, Robert A. Andersen, Mary Alice Coffroth, David W Galbraith

Research output: Contribution to journalArticle

137 Citations (Scopus)

Abstract

Using flow cytometric analysis of fluorescence, we measured the genome sizes of 18 cultured "free-living" species and 29 Symbiodinium spp. isolates cultured from stony corals, gorgonians, anemones, jellyfish, and giant clams. Genome size directly correlated with cell size, as documented previously for most eukaryotic cell lines. Among the smallest of dinoflagellates, Symbiodinium spp. (6-15 μm) possessed the lowest DNA content that we measured (1.5-4.8 pg · cell-1). Bloom-forming or potentially harmful species in the genera Alexandrium, Karenia, Pfiesteria, and Prorocentrum possessed genomes approximately 2 to 50 times larger in size. A phylogenetic analysis indicated that genome/cell size has apparently increased and decreased repeatedly during the evolution of dinoflagellates. In contrast, genome sizes were relatively consistent across distantly and closely related Symbiodinium spp. This may be the product of intracellular host habitats imposing strong selective pressures that have restricted symbiont size.

Original languageEnglish (US)
Pages (from-to)880-886
Number of pages7
JournalJournal of Phycology
Volume41
Issue number4
DOIs
StatePublished - Aug 2005

Fingerprint

Symbiodinium
dinoflagellate
genome
DNA
Pfiesteria
Prorocentrum
Alexandrium
Scyphozoa
cells
clams
symbionts
jellyfish
eukaryotic cells
cell lines
Miozoa
symbiont
fluorescence
coral
algal bloom
phylogeny

Keywords

  • C-values
  • Dinoflagellates
  • Genome size
  • Symbiodinium
  • Zooxanthellae

ASJC Scopus subject areas

  • Aquatic Science
  • Plant Science

Cite this

Symbiodinium (Pyrrhophyta) genome sizes (DNA content) are smallest among dinoflagellates. / LaJeunesse, Todd C.; Lambert, Georgina; Andersen, Robert A.; Coffroth, Mary Alice; Galbraith, David W.

In: Journal of Phycology, Vol. 41, No. 4, 08.2005, p. 880-886.

Research output: Contribution to journalArticle

LaJeunesse, Todd C. ; Lambert, Georgina ; Andersen, Robert A. ; Coffroth, Mary Alice ; Galbraith, David W. / Symbiodinium (Pyrrhophyta) genome sizes (DNA content) are smallest among dinoflagellates. In: Journal of Phycology. 2005 ; Vol. 41, No. 4. pp. 880-886.
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