Synergid cell death in Arabidopsis is triggered following direct interaction with the pollen tube

Linda Sandaklie-Nikolova, Ravishankar Palanivelu, Edward J. King, Gregory P. Copenhaver, Gary N. Drews

Research output: Contribution to journalArticle

74 Citations (Scopus)

Abstract

During angiosperm reproduction, one of the two synergid cells within the female gametophyte undergoes cell death prior to fertilization. The pollen tube enters the female gametophyte by growing into the synergid cell that undergoes cell death and releases its two sperm cells within the degenerating synergid cytoplasm to effect double fertilization. In Arabidopsis (Arabidopsis thaliana) and many other species, synergid cell death is dependent upon pollination. However, the mechanism by which the pollen tube causes synergid cell death is not understood. As a first step toward understanding this mechanism, we defined the temporal relationship between pollen tube arrival at the female gametophyte and synergid cell death in Arabidopsis. Using confocal laser scanning microscopy, light microscopy, transmission electron microscopy, and real-time observation of these two events in vitro, we demonstrate that synergid cell death initiates after the pollen tube arrives at the female gametophyte but before pollen tube discharge. Our results support a model in which a signaling cascade triggered by pollen tube-synergid cell contact induces synergid cell death in Arabidopsis.

Original languageEnglish (US)
Pages (from-to)1753-1762
Number of pages10
JournalPlant Physiology
Volume144
Issue number4
DOIs
StatePublished - Aug 2007

Fingerprint

Pollen Tube
pollen tubes
Arabidopsis
cell death
Cell Death
fertilization (reproduction)
Fertilization
cells
Angiosperms
Pollination
confocal laser scanning microscopy
Transmission Electron Microscopy
Confocal Microscopy
Reproduction
transmission electron microscopy
Spermatozoa
light microscopy
Microscopy
Angiospermae
pollination

ASJC Scopus subject areas

  • Plant Science

Cite this

Synergid cell death in Arabidopsis is triggered following direct interaction with the pollen tube. / Sandaklie-Nikolova, Linda; Palanivelu, Ravishankar; King, Edward J.; Copenhaver, Gregory P.; Drews, Gary N.

In: Plant Physiology, Vol. 144, No. 4, 08.2007, p. 1753-1762.

Research output: Contribution to journalArticle

Sandaklie-Nikolova, Linda ; Palanivelu, Ravishankar ; King, Edward J. ; Copenhaver, Gregory P. ; Drews, Gary N. / Synergid cell death in Arabidopsis is triggered following direct interaction with the pollen tube. In: Plant Physiology. 2007 ; Vol. 144, No. 4. pp. 1753-1762.
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