Synergy between an antibody and CD8+ cells in eliminating an established tumor

Ljiljana V. Vasović, Ruben Dyall, Raphael A. Clynes, Jeffrey V. Ravetch, Janko Nikolić-Žugić

Research output: Contribution to journalArticle

29 Scopus citations

Abstract

We investigated the effector mechanisms operating during the rejection of a transplantable solid lymphoma E.G7 (H-2b) which expresses the gene encoding chicken ovalbumin (OVA). Anti-OVA cytotoxic T lymphocytes (CTL) completely and specifically protected animals from the onset of, but could not eradicate established, E.G7 tumors. The growth of the same lymphoma was also effectively prevented by the antibody GK1.5, whose target molecule, CD4, was expressed on E.G7 cells in vivo. Furthermore, GK1.5 was able to eradicate established solid E.G7 tumors. GK1.5-mediated tumor elimination was due to its antitumor activity, and not to the elimination of regulatory CD4+ cells, based on unimpaired tumor growth in the absence of GK1.5 in animals that genetically lack CD4 T cells. In vitro, GK1.5 did not kill tumor cells: complement activation or apoptosis induction were not evident. In vivo, GK1.5-mediated tumor regression did not depend on natural killer cells, but it absolutely required CD8+ cells and intact Fcγ receptor. We conclude that, in the E.G7 model, the collaboration of antibody and CTL immunity was crucial for the successful immunotherapy of established tumors. The mechanism of this collaboration is discussed.

Original languageEnglish (US)
Pages (from-to)374-382
Number of pages9
JournalEuropean Journal of Immunology
Volume27
Issue number2
DOIs
StatePublished - 1997

Keywords

  • Antibody
  • Cytotoxic T lymphocyte
  • Lymphoma
  • Tumor regression

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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