Synthesis and resistance to enzymic hydrolysis of stereochemically-defined phosphonate and thiophosphate analogues of P1,P4-bis(5'-adenosyl) tetraphosphate.

G. M. Blackburn, G. E. Taylor, G. R. Thatcher, M. Prescott, A. G. McLennan

Research output: Contribution to journalArticlepeer-review

48 Scopus citations

Abstract

Novel analogues of P1,P4-bis(5'-adenosyl) tetraphosphate, Ap4A (1), have been prepared with sulphur substituents at P1 and P4 and either oxygen or methylene bridges at the P2,P3-position. Separation of three isomers of the ApspCH2ppsA species has been achieved by a combination of mplc and hplc and the Rp,Rp, Rp,Sp, and Sp,Sp diastereoisomers identified on the basis of selective enzymatic hydrolysis using snake venom phosphodiesterase. Each of these three isomers is a strong competitive inhibitor of the specific Ap4Aase from Artemia and is highly resistant to the asymmetric cleavage normally catalysed by this enzyme.

Original languageEnglish (US)
Pages (from-to)6991-7004
Number of pages14
JournalNucleic acids research
Volume15
Issue number17
DOIs
StatePublished - Sep 11 1987
Externally publishedYes

ASJC Scopus subject areas

  • Genetics

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