The solid phase synthesis of highly purified [Leu5] enkephalin and of seven derivatives including [Ala2,Leu5]-, [Ser2,Leu5]-, [Ser3,Leu5]-, [Aba2,Leu5]-, and [des-Gly2(3),Leu5]enkephalins are reported, and their morphine-like activities in neuroblastoma x glioma cell homogenates were measured. Changes at the 2, 3, and 5 positions of the enkephalin provided analogues which were all less active than [Leu5] enkephalin. The results are discussed in terms of recently suggested conformational structures for the enkephalin peptides. No melanocyte stimulating activity was observed for [Leu5] enkephalin, [Ala2,Leu5]enkephalin, or [Ser2,Leu5] enkephalin.
|Original language||English (US)|
|Number of pages||7|
|Journal||Biochemical and Biophysical Research Communications|
|State||Published - May 9 1977|
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology