Synthetic matrix metalloproteinase inhibitor, BB-94, inhibits the invasion of neoplastic human prostate cells in a mouse model

J. David Knox, Louis Bretton, Tamara Lynch, G. Tim Bowden, Raymond B Nagle

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

BACKGROUND. It has been suggested that increased metalloproteinase activity is a critical event in neoplastic progression leading to the initiation of local invasion and ultimately to the dissemination of neoplastic cells. This has led to an interest in testing the ability of metalloproteinase inhibitors to prevent the progression of carcinoma in situ into invasive and, therefore, more malignant tumors. One such agent is the synthetic matrix metalloproteinase inhibitor, BB-94. METHODS. The effect of BB-94 on the intrinsic invasive potential of matrilysin-transfected Du-145 cells was evaluated by an in vitro invasion assay. In addition a diaphragm invasion model, which provides an easily oriented structure in which the earliest penetration of the basal lamina can be observed, was used to investigate the effect of BB-94 on the invasion and growth of tumors formed by these cells when injected into S.C.I.D. mice. RESULTS. The synthetic matrix metalloproteinase inhibitor, BB-94, was shown to effectively inhibit the invasion of matrigel and murine diaphragm. CONCLUSIONS. Metalloproteinase inhibitors, such as BB-94, that are able to limit tumor growth, and local invasion, may decrease the invasion of invasive carcinomas.

Original languageEnglish (US)
Pages (from-to)248-254
Number of pages7
JournalProstate
Volume35
Issue number4
DOIs
StatePublished - Jun 1 1998

Fingerprint

Matrix Metalloproteinase Inhibitors
Prostate
Metalloproteases
Diaphragm
Matrix Metalloproteinase 7
Neoplasms
SCID Mice
Carcinoma in Situ
Growth
Basement Membrane
HSCH2CH(CH2CH(CH3)2)CO-Phe-Ala-NH2
batimastat
Carcinoma

Keywords

  • Batimastat
  • DU-145
  • Matrigel
  • Matrilysin

ASJC Scopus subject areas

  • Urology

Cite this

Synthetic matrix metalloproteinase inhibitor, BB-94, inhibits the invasion of neoplastic human prostate cells in a mouse model. / Knox, J. David; Bretton, Louis; Lynch, Tamara; Bowden, G. Tim; Nagle, Raymond B.

In: Prostate, Vol. 35, No. 4, 01.06.1998, p. 248-254.

Research output: Contribution to journalArticle

Knox, J. David ; Bretton, Louis ; Lynch, Tamara ; Bowden, G. Tim ; Nagle, Raymond B. / Synthetic matrix metalloproteinase inhibitor, BB-94, inhibits the invasion of neoplastic human prostate cells in a mouse model. In: Prostate. 1998 ; Vol. 35, No. 4. pp. 248-254.
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