Systemic human CD34<sup>+</sup> cells populate the brain and activate host mechanisms to counteract nigrostriatal degeneration

Mando J. Corenblum, Andrew J. Flores, Michael Badowski, David T. Harris, Lalitha Madhavan

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Aim: Here we investigated the neuroprotective potential of systemic CD34<sup>+</sup> human cord blood cells (hCBCs) in a 6-hydroxydopamine rat model of Parkinson's disease. Methods: Purified CD34<sup>+</sup> hCBCs were intravenously administered to rats subjected to 6-hydroxydopamine 24 h earlier, and behavioral and immunohistological analysis performed. Results: CD34<sup>+</sup> hCBC administration significantly prevented host nigrostriatal degeneration inducing behavioral recovery in treated rats. Although donor hCBCs did not differentiate into neural phenotypes, they stimulated the production of new neuroblasts and angiogenesis, and reduced gliosis in recipient animals. Importantly, surviving donor hCBCs were identified, and their tissue distribution pattern correlated with the observed therapeutic effects. Conclusion: Peripherally applied CD34<sup>+</sup> hCBCs can migrate into brain tissues and elicit host-based protective mechanisms to support the survival of midbrain dopamine neurons.

Original languageEnglish (US)
Pages (from-to)563-577
Number of pages15
JournalRegenerative Medicine
Volume10
Issue number5
DOIs
StatePublished - Aug 1 2015

Fingerprint

Fetal Blood
Brain
Blood Cells
Blood
Rats
Oxidopamine
Tissue
Gliosis
Dopaminergic Neurons
Therapeutic Uses
Tissue Distribution
Mesencephalon
Neurons
Parkinson Disease
Animals
Cells
Phenotype
Recovery

Keywords

  • 6-OHDA
  • angiogenesis
  • CD34<sup>+</sup> mononuclear cells
  • cord blood
  • neurogenesis
  • neuroprotection
  • Parkinson's disease

ASJC Scopus subject areas

  • Biomedical Engineering
  • Embryology

Cite this

Systemic human CD34<sup>+</sup> cells populate the brain and activate host mechanisms to counteract nigrostriatal degeneration. / Corenblum, Mando J.; Flores, Andrew J.; Badowski, Michael; Harris, David T.; Madhavan, Lalitha.

In: Regenerative Medicine, Vol. 10, No. 5, 01.08.2015, p. 563-577.

Research output: Contribution to journalArticle

@article{3f9da71918604fa6a365591643d625b3,
title = "Systemic human CD34+ cells populate the brain and activate host mechanisms to counteract nigrostriatal degeneration",
abstract = "Aim: Here we investigated the neuroprotective potential of systemic CD34+ human cord blood cells (hCBCs) in a 6-hydroxydopamine rat model of Parkinson's disease. Methods: Purified CD34+ hCBCs were intravenously administered to rats subjected to 6-hydroxydopamine 24 h earlier, and behavioral and immunohistological analysis performed. Results: CD34+ hCBC administration significantly prevented host nigrostriatal degeneration inducing behavioral recovery in treated rats. Although donor hCBCs did not differentiate into neural phenotypes, they stimulated the production of new neuroblasts and angiogenesis, and reduced gliosis in recipient animals. Importantly, surviving donor hCBCs were identified, and their tissue distribution pattern correlated with the observed therapeutic effects. Conclusion: Peripherally applied CD34+ hCBCs can migrate into brain tissues and elicit host-based protective mechanisms to support the survival of midbrain dopamine neurons.",
keywords = "6-OHDA, angiogenesis, CD34<sup>+</sup> mononuclear cells, cord blood, neurogenesis, neuroprotection, Parkinson's disease",
author = "Corenblum, {Mando J.} and Flores, {Andrew J.} and Michael Badowski and Harris, {David T.} and Lalitha Madhavan",
year = "2015",
month = "8",
day = "1",
doi = "10.2217/rme.15.32",
language = "English (US)",
volume = "10",
pages = "563--577",
journal = "Regenerative Medicine",
issn = "1746-0751",
publisher = "Future Medicine Ltd.",
number = "5",

}

TY - JOUR

T1 - Systemic human CD34+ cells populate the brain and activate host mechanisms to counteract nigrostriatal degeneration

AU - Corenblum, Mando J.

AU - Flores, Andrew J.

AU - Badowski, Michael

AU - Harris, David T.

AU - Madhavan, Lalitha

PY - 2015/8/1

Y1 - 2015/8/1

N2 - Aim: Here we investigated the neuroprotective potential of systemic CD34+ human cord blood cells (hCBCs) in a 6-hydroxydopamine rat model of Parkinson's disease. Methods: Purified CD34+ hCBCs were intravenously administered to rats subjected to 6-hydroxydopamine 24 h earlier, and behavioral and immunohistological analysis performed. Results: CD34+ hCBC administration significantly prevented host nigrostriatal degeneration inducing behavioral recovery in treated rats. Although donor hCBCs did not differentiate into neural phenotypes, they stimulated the production of new neuroblasts and angiogenesis, and reduced gliosis in recipient animals. Importantly, surviving donor hCBCs were identified, and their tissue distribution pattern correlated with the observed therapeutic effects. Conclusion: Peripherally applied CD34+ hCBCs can migrate into brain tissues and elicit host-based protective mechanisms to support the survival of midbrain dopamine neurons.

AB - Aim: Here we investigated the neuroprotective potential of systemic CD34+ human cord blood cells (hCBCs) in a 6-hydroxydopamine rat model of Parkinson's disease. Methods: Purified CD34+ hCBCs were intravenously administered to rats subjected to 6-hydroxydopamine 24 h earlier, and behavioral and immunohistological analysis performed. Results: CD34+ hCBC administration significantly prevented host nigrostriatal degeneration inducing behavioral recovery in treated rats. Although donor hCBCs did not differentiate into neural phenotypes, they stimulated the production of new neuroblasts and angiogenesis, and reduced gliosis in recipient animals. Importantly, surviving donor hCBCs were identified, and their tissue distribution pattern correlated with the observed therapeutic effects. Conclusion: Peripherally applied CD34+ hCBCs can migrate into brain tissues and elicit host-based protective mechanisms to support the survival of midbrain dopamine neurons.

KW - 6-OHDA

KW - angiogenesis

KW - CD34<sup>+</sup> mononuclear cells

KW - cord blood

KW - neurogenesis

KW - neuroprotection

KW - Parkinson's disease

UR - http://www.scopus.com/inward/record.url?scp=84938574030&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84938574030&partnerID=8YFLogxK

U2 - 10.2217/rme.15.32

DO - 10.2217/rme.15.32

M3 - Article

C2 - 26237701

AN - SCOPUS:84938574030

VL - 10

SP - 563

EP - 577

JO - Regenerative Medicine

JF - Regenerative Medicine

SN - 1746-0751

IS - 5

ER -