T-cell receptor Vβ8.1 peptide reduces coxsackievirus-induced cardiopathology in aged mice

Ramón Tomás Sepúlveda, John Jacob Marchalonis, Ronald R Watson

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Viral myocarditis is an important cause of heart failure and cardiomyopathy. Immunosenescence, characterized by a dramatic reduction in immune responsiveness, can increase susceptibility to cardiopathology from viral infections. The T-cell receptor (TCR) Vβ8.1 peptide, a 16-merpeptide, has shown immunoregulatingand immunostimulating effects in viral-induced immunodeficiency. In our study, 18-mo-old C57BI/6 female mice were treated twice with TCR Vβ8.1 peptide and 10 d before sacrifice were injected ip with coxsackievirus B3. Cardiac histopathology was assessed for lesion severity. Splenocyte cytokine production (interleukin-2, -4, -6, interferon-γ) and heart viral titers were determined. Our data suggest that immunosenescence suppressed both T helper (Th1) and Th2 cytokine production and that treatment with TCR Vβ8.1 peptide induced cytokine stimulation close to levels seen in young mice. Nontreated aged mice developed some degree of myocarditis (75% mild and 25% severe), whereas only 35% of the peptide-treated aged group developed cardiopathology, with 25% being mild and 10% severe. Heart tissue from nontreated aged mice infected with coxsackievirus had a higher viral titer than hearts of aged mice equally infected but treated with the peptide. In conclusion, TCR Vβ8.1 peptide induced immunoregulation, and inhibited or reduced coxsackievirus B3-induced cardiopathology in aged mice.

Original languageEnglish (US)
Pages (from-to)21-28
Number of pages8
JournalCardiovascular Toxicology
Volume5
Issue number1
StatePublished - 2005

Fingerprint

Enterovirus
T-Cell Antigen Receptor
Peptides
Myocarditis
Cytokines
Virus Diseases
Cardiomyopathies
Interleukin-4
Interferons
Interleukin-2
Heart Failure
Tissue

Keywords

  • Age
  • Cardiopathology
  • Coxsackievirus
  • Cytokines
  • Immunosenescence
  • Myocarditis
  • Peptide therapy
  • TCR
  • Th1
  • Th2

ASJC Scopus subject areas

  • Toxicology
  • Cardiology and Cardiovascular Medicine

Cite this

T-cell receptor Vβ8.1 peptide reduces coxsackievirus-induced cardiopathology in aged mice. / Sepúlveda, Ramón Tomás; Marchalonis, John Jacob; Watson, Ronald R.

In: Cardiovascular Toxicology, Vol. 5, No. 1, 2005, p. 21-28.

Research output: Contribution to journalArticle

Sepúlveda, Ramón Tomás ; Marchalonis, John Jacob ; Watson, Ronald R. / T-cell receptor Vβ8.1 peptide reduces coxsackievirus-induced cardiopathology in aged mice. In: Cardiovascular Toxicology. 2005 ; Vol. 5, No. 1. pp. 21-28.
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