T-DNA insertion mutagenesis in Arabidopsis: Going back and forth

Ricardo Azpiroz-Leehan, Kenneth A. Feldmann

Research output: Contribution to journalReview article

239 Scopus citations

Abstract

T-DNA insertion mutagenesis in Arabidopsis has been instrumental in advancing our knowledge of the physiolagy, biochemistry and development of plants. In just a few years plant biology, the late starter of molecular genetics, has placed itself at the forefront of science in large measure as a result of the more than 20000 T-DNA transformants that have been generated in Arabidopsis. To date, approximately 4000 mutants have been identified and more than 40 genes have been isolated via the T-DNA tag. The large population of transformed lines is now being subjected to a PCR-based reverse genetics strategy to identify new insertion mutants in sequences of interest.

Original languageEnglish (US)
Pages (from-to)152-156
Number of pages5
JournalTrends in Genetics
Volume13
Issue number4
DOIs
StatePublished - Apr 1 1997

ASJC Scopus subject areas

  • Genetics

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