T-DNA insertion mutagenesis in Arabidopsis: Mutational spectrum

Research output: Contribution to journalArticle

407 Citations (Scopus)

Abstract

More than 8000 transformants of Arabidopsis have been generated by treating germinating seeds with cultures of Agrobacterium tumefaciens. Genetic characterization of a subset of the transformants indicates that they contain an average of 1.4 inserts each, as assayed by kanamycin resistance. Molecular analysis shows that the inserts are predominantly concatamers of T-DNAs arranged as direct and inverted repeats. More recently these 8000 lines have been screened under a variety of growth conditions for visible alterations in phenotype. More than 1000 putative mutants were observed during the application of these screening procedures. These mutants fall into several general classes: seedling-lethals, size variants, pigment, embryo-defective, reduced-fertility, dramatic (morphological), and physiological. The majority of the mutants (88%) segregate in a Mendelian manner for the mutant phenotype. An analysis of approximately 50 mutants in this group shows that >80% are tagged with a functional insert. The wide spectrum of mutants observed suggests that it may be feasible to develop a comprehensive collection of mutant lines in which each gene is tagged by a T-DNA insertion.

Original languageEnglish (US)
Pages (from-to)71-82
Number of pages12
JournalPlant Journal
Volume1
Issue number1
StatePublished - 1991

Fingerprint

Arabidopsis
mutagenesis
Mutagenesis
Kanamycin Resistance
Phenotype
mutants
Agrobacterium tumefaciens
Nucleic Acid Repetitive Sequences
DNA
Seedlings
Fertility
Seeds
Embryonic Structures
Growth
Genes
phenotype
kanamycin
T-DNA
Agrobacterium radiobacter
lethal genes

ASJC Scopus subject areas

  • Plant Science

Cite this

T-DNA insertion mutagenesis in Arabidopsis : Mutational spectrum. / Feldmann, Kenneth A.

In: Plant Journal, Vol. 1, No. 1, 1991, p. 71-82.

Research output: Contribution to journalArticle

@article{9629b23481554bab93f3f56c4d92085a,
title = "T-DNA insertion mutagenesis in Arabidopsis: Mutational spectrum",
abstract = "More than 8000 transformants of Arabidopsis have been generated by treating germinating seeds with cultures of Agrobacterium tumefaciens. Genetic characterization of a subset of the transformants indicates that they contain an average of 1.4 inserts each, as assayed by kanamycin resistance. Molecular analysis shows that the inserts are predominantly concatamers of T-DNAs arranged as direct and inverted repeats. More recently these 8000 lines have been screened under a variety of growth conditions for visible alterations in phenotype. More than 1000 putative mutants were observed during the application of these screening procedures. These mutants fall into several general classes: seedling-lethals, size variants, pigment, embryo-defective, reduced-fertility, dramatic (morphological), and physiological. The majority of the mutants (88{\%}) segregate in a Mendelian manner for the mutant phenotype. An analysis of approximately 50 mutants in this group shows that >80{\%} are tagged with a functional insert. The wide spectrum of mutants observed suggests that it may be feasible to develop a comprehensive collection of mutant lines in which each gene is tagged by a T-DNA insertion.",
author = "Feldmann, {Kenneth A}",
year = "1991",
language = "English (US)",
volume = "1",
pages = "71--82",
journal = "Plant Journal",
issn = "0960-7412",
publisher = "Wiley-Blackwell",
number = "1",

}

TY - JOUR

T1 - T-DNA insertion mutagenesis in Arabidopsis

T2 - Mutational spectrum

AU - Feldmann, Kenneth A

PY - 1991

Y1 - 1991

N2 - More than 8000 transformants of Arabidopsis have been generated by treating germinating seeds with cultures of Agrobacterium tumefaciens. Genetic characterization of a subset of the transformants indicates that they contain an average of 1.4 inserts each, as assayed by kanamycin resistance. Molecular analysis shows that the inserts are predominantly concatamers of T-DNAs arranged as direct and inverted repeats. More recently these 8000 lines have been screened under a variety of growth conditions for visible alterations in phenotype. More than 1000 putative mutants were observed during the application of these screening procedures. These mutants fall into several general classes: seedling-lethals, size variants, pigment, embryo-defective, reduced-fertility, dramatic (morphological), and physiological. The majority of the mutants (88%) segregate in a Mendelian manner for the mutant phenotype. An analysis of approximately 50 mutants in this group shows that >80% are tagged with a functional insert. The wide spectrum of mutants observed suggests that it may be feasible to develop a comprehensive collection of mutant lines in which each gene is tagged by a T-DNA insertion.

AB - More than 8000 transformants of Arabidopsis have been generated by treating germinating seeds with cultures of Agrobacterium tumefaciens. Genetic characterization of a subset of the transformants indicates that they contain an average of 1.4 inserts each, as assayed by kanamycin resistance. Molecular analysis shows that the inserts are predominantly concatamers of T-DNAs arranged as direct and inverted repeats. More recently these 8000 lines have been screened under a variety of growth conditions for visible alterations in phenotype. More than 1000 putative mutants were observed during the application of these screening procedures. These mutants fall into several general classes: seedling-lethals, size variants, pigment, embryo-defective, reduced-fertility, dramatic (morphological), and physiological. The majority of the mutants (88%) segregate in a Mendelian manner for the mutant phenotype. An analysis of approximately 50 mutants in this group shows that >80% are tagged with a functional insert. The wide spectrum of mutants observed suggests that it may be feasible to develop a comprehensive collection of mutant lines in which each gene is tagged by a T-DNA insertion.

UR - http://www.scopus.com/inward/record.url?scp=0002724395&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0002724395&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:0002724395

VL - 1

SP - 71

EP - 82

JO - Plant Journal

JF - Plant Journal

SN - 0960-7412

IS - 1

ER -