Taming of a poison: Biosynthesis of the NiFE-hydrogenase cyanide ligands

Stefanie Reissmann, Elisabeth Hochleitner, Haofan Wang, Athanasios Paschos, Friedrich Lottspeich, Richard S Glass, August Böck

Research output: Contribution to journalArticle

162 Citations (Scopus)

Abstract

NiFe-hydrogenases have an Ni-Fe site in which the iron has one CO and two CN groups as ligands. Synthesis of the CN ligands requires the activity of two hydrogenase maturation proteins: HypF and HypE. HypF is a carbamoyl-transferase that transfers the carbamoyl moiety of carbamoyladenylate to the COOH-terminal cysteine of HypE and thus forms an enzyme-thiocarbamate. HypE dehydrates the S-carbamoyl moiety in an adenosine triphosphate-dependent process to yield the enzyme thiocyanate. Chemical model reactions corroborate the feasibility of this unprecedented biosynthetic route and show that thiocyanates can donate CN to iron. This finding underscores a striking parallel between biochemistry and organometallic chemistry in the formation of an iron-cyano complex.

Original languageEnglish (US)
Pages (from-to)1067-1070
Number of pages4
JournalScience
Volume299
Issue number5609
DOIs
StatePublished - Feb 14 2003

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Hydrogenase
Poisons
Cyanides
Iron
Ligands
Carboxyl and Carbamoyl Transferases
Thiocyanates
Thiocarbamates
Chemical Models
Enzymes
Carbon Monoxide
Biochemistry
Cysteine
Adenosine Triphosphate
Proteins

ASJC Scopus subject areas

  • General

Cite this

Reissmann, S., Hochleitner, E., Wang, H., Paschos, A., Lottspeich, F., Glass, R. S., & Böck, A. (2003). Taming of a poison: Biosynthesis of the NiFE-hydrogenase cyanide ligands. Science, 299(5609), 1067-1070. https://doi.org/10.1126/science.1080972

Taming of a poison : Biosynthesis of the NiFE-hydrogenase cyanide ligands. / Reissmann, Stefanie; Hochleitner, Elisabeth; Wang, Haofan; Paschos, Athanasios; Lottspeich, Friedrich; Glass, Richard S; Böck, August.

In: Science, Vol. 299, No. 5609, 14.02.2003, p. 1067-1070.

Research output: Contribution to journalArticle

Reissmann, S, Hochleitner, E, Wang, H, Paschos, A, Lottspeich, F, Glass, RS & Böck, A 2003, 'Taming of a poison: Biosynthesis of the NiFE-hydrogenase cyanide ligands', Science, vol. 299, no. 5609, pp. 1067-1070. https://doi.org/10.1126/science.1080972
Reissmann S, Hochleitner E, Wang H, Paschos A, Lottspeich F, Glass RS et al. Taming of a poison: Biosynthesis of the NiFE-hydrogenase cyanide ligands. Science. 2003 Feb 14;299(5609):1067-1070. https://doi.org/10.1126/science.1080972
Reissmann, Stefanie ; Hochleitner, Elisabeth ; Wang, Haofan ; Paschos, Athanasios ; Lottspeich, Friedrich ; Glass, Richard S ; Böck, August. / Taming of a poison : Biosynthesis of the NiFE-hydrogenase cyanide ligands. In: Science. 2003 ; Vol. 299, No. 5609. pp. 1067-1070.
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