Target cell susceptibility to immune lysis and expression of MHC antigens are independent of position in the cell cycle

David T. Harris, R. P. Sekaly

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

We have investigated the effects of progression through the mitotic cell cycle upon the tumor (P815) cell line's expression of H-2 antigens and susceptibility to immune cytolysis. By using a combination of DNA quantitation by Hoechst 33342 staining and fluorescence-activated cell sorter analysis we were able to obtained pure populations of G1, S, and G2 + M cells for study. Furthermore, by employing a fluorescence quenching technique utilizing bromodeoxyuridine substitution we were able to monitor the times required for the P815 cells to transit each of the various phases. By using mAb and cloned CTL populations with well-defined specificities we observed that neither H-2 antigen expression nor lytic susceptibility to mAb plus complement or CTL fluctuated with cell cycle progression. Each of the three major MHC loci (H-2K(d), -D(d), and L(d)) was analyzed. These results demonstrated that expression of H-2 antigens and susceptibility to immune lysis were independent of a cell's position in the cell cycle.

Original languageEnglish (US)
Pages (from-to)40-44
Number of pages5
JournalJournal of Immunology
Volume133
Issue number1
StatePublished - 1984
Externally publishedYes

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H-2 Antigens
Cell Cycle
Antigens
Fluorescence
Bromodeoxyuridine
Tumor Cell Line
Population
Staining and Labeling
DNA

ASJC Scopus subject areas

  • Immunology

Cite this

Target cell susceptibility to immune lysis and expression of MHC antigens are independent of position in the cell cycle. / Harris, David T.; Sekaly, R. P.

In: Journal of Immunology, Vol. 133, No. 1, 1984, p. 40-44.

Research output: Contribution to journalArticle

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