Targeted ablation of plasma membrane Ca2+-ATPase (PMCA) 1 and 4 indicates a major housekeeping function for PMCA1 and a critical role in hyperactivated sperm motility and male fertility for PMCA4

Gbolahan W. Okunade, Marian L. Miller, Gail J. Pyne, Roy L. Sutliff, Kyle T. O'Connor, Jonathan C. Neumann, Anastasia Andringa, Daniel A. Miller, Vikram Prasad, Thomas C Doetschman, Richard J. Paul, Gary E. Shull

Research output: Contribution to journalArticle

227 Citations (Scopus)

Abstract

The relative importance of plasma membrane Ca2+-ATPase (PMCA) 1 and PMCA4 was assessed in mice carrying null mutations in their genes (Atp2b1 and Atp2b4). Loss of both copies of the gene encoding PMCA1 caused embryolethality, whereas heterozygous mutants had no overt disease phenotype. Despite widespread and abundant expression of PMCA4, PMCA4 null (Pmca4 -/-) mutants exhibited no embryolethality and appeared outwardly normal. Loss of PMCA4 impaired phasic contractions and caused apoptosis in portal vein smooth muscle in vitro; however, this phenotype was dependent on the mouse strain being employed. Pmca4-/- mice on a Black Swiss background did not exhibit the phenotype unless they also carried a null mutation in one copy of the Pmca1 gene. Pmca4-/- male mice were infertile but had normal spermatogenesis and mating behavior. Pmca4 -/- sperm that had not undergone capacitation exhibited normal motility but could not achieve hyperactivated motility needed to traverse the female genital tract. Ultrastructure of the motility apparatus in Pmca4 -/- sperm tails was normal, but an increased incidence of mitochondrial condensation indicated Ca2+ overload. Immunoblotting and immuno-histochemistry showed that PMCA4 is the most abundant isoform in testis and sperm and that it is localized to the principle piece of the sperm tail, which is also the location of the major Ca2+ channel (CatSper) required for sperm motility. These results are consistent with an essential housekeeping or developmental function for PMCA1, but not PMCA4, and show that PMCA4 expression in the principle piece of the sperm tail is essential for hyperactivated motility and male fertility.

Original languageEnglish (US)
Pages (from-to)33742-33750
Number of pages9
JournalJournal of Biological Chemistry
Volume279
Issue number32
DOIs
StatePublished - Aug 6 2004
Externally publishedYes

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Housekeeping
Calcium-Transporting ATPases
Sperm Motility
Cell membranes
Sperm Tail
Ablation
Fertility
Genes
Cell Membrane
Gene encoding
Phenotype
Muscle
Spermatozoa
Condensation
Protein Isoforms
Apoptosis
Mutation
Spermatogenesis
Portal Vein
Immunoblotting

ASJC Scopus subject areas

  • Biochemistry

Cite this

Targeted ablation of plasma membrane Ca2+-ATPase (PMCA) 1 and 4 indicates a major housekeeping function for PMCA1 and a critical role in hyperactivated sperm motility and male fertility for PMCA4. / Okunade, Gbolahan W.; Miller, Marian L.; Pyne, Gail J.; Sutliff, Roy L.; O'Connor, Kyle T.; Neumann, Jonathan C.; Andringa, Anastasia; Miller, Daniel A.; Prasad, Vikram; Doetschman, Thomas C; Paul, Richard J.; Shull, Gary E.

In: Journal of Biological Chemistry, Vol. 279, No. 32, 06.08.2004, p. 33742-33750.

Research output: Contribution to journalArticle

Okunade, Gbolahan W. ; Miller, Marian L. ; Pyne, Gail J. ; Sutliff, Roy L. ; O'Connor, Kyle T. ; Neumann, Jonathan C. ; Andringa, Anastasia ; Miller, Daniel A. ; Prasad, Vikram ; Doetschman, Thomas C ; Paul, Richard J. ; Shull, Gary E. / Targeted ablation of plasma membrane Ca2+-ATPase (PMCA) 1 and 4 indicates a major housekeeping function for PMCA1 and a critical role in hyperactivated sperm motility and male fertility for PMCA4. In: Journal of Biological Chemistry. 2004 ; Vol. 279, No. 32. pp. 33742-33750.
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abstract = "The relative importance of plasma membrane Ca2+-ATPase (PMCA) 1 and PMCA4 was assessed in mice carrying null mutations in their genes (Atp2b1 and Atp2b4). Loss of both copies of the gene encoding PMCA1 caused embryolethality, whereas heterozygous mutants had no overt disease phenotype. Despite widespread and abundant expression of PMCA4, PMCA4 null (Pmca4 -/-) mutants exhibited no embryolethality and appeared outwardly normal. Loss of PMCA4 impaired phasic contractions and caused apoptosis in portal vein smooth muscle in vitro; however, this phenotype was dependent on the mouse strain being employed. Pmca4-/- mice on a Black Swiss background did not exhibit the phenotype unless they also carried a null mutation in one copy of the Pmca1 gene. Pmca4-/- male mice were infertile but had normal spermatogenesis and mating behavior. Pmca4 -/- sperm that had not undergone capacitation exhibited normal motility but could not achieve hyperactivated motility needed to traverse the female genital tract. Ultrastructure of the motility apparatus in Pmca4 -/- sperm tails was normal, but an increased incidence of mitochondrial condensation indicated Ca2+ overload. Immunoblotting and immuno-histochemistry showed that PMCA4 is the most abundant isoform in testis and sperm and that it is localized to the principle piece of the sperm tail, which is also the location of the major Ca2+ channel (CatSper) required for sperm motility. These results are consistent with an essential housekeeping or developmental function for PMCA1, but not PMCA4, and show that PMCA4 expression in the principle piece of the sperm tail is essential for hyperactivated motility and male fertility.",
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T1 - Targeted ablation of plasma membrane Ca2+-ATPase (PMCA) 1 and 4 indicates a major housekeeping function for PMCA1 and a critical role in hyperactivated sperm motility and male fertility for PMCA4

AU - Okunade, Gbolahan W.

AU - Miller, Marian L.

AU - Pyne, Gail J.

AU - Sutliff, Roy L.

AU - O'Connor, Kyle T.

AU - Neumann, Jonathan C.

AU - Andringa, Anastasia

AU - Miller, Daniel A.

AU - Prasad, Vikram

AU - Doetschman, Thomas C

AU - Paul, Richard J.

AU - Shull, Gary E.

PY - 2004/8/6

Y1 - 2004/8/6

N2 - The relative importance of plasma membrane Ca2+-ATPase (PMCA) 1 and PMCA4 was assessed in mice carrying null mutations in their genes (Atp2b1 and Atp2b4). Loss of both copies of the gene encoding PMCA1 caused embryolethality, whereas heterozygous mutants had no overt disease phenotype. Despite widespread and abundant expression of PMCA4, PMCA4 null (Pmca4 -/-) mutants exhibited no embryolethality and appeared outwardly normal. Loss of PMCA4 impaired phasic contractions and caused apoptosis in portal vein smooth muscle in vitro; however, this phenotype was dependent on the mouse strain being employed. Pmca4-/- mice on a Black Swiss background did not exhibit the phenotype unless they also carried a null mutation in one copy of the Pmca1 gene. Pmca4-/- male mice were infertile but had normal spermatogenesis and mating behavior. Pmca4 -/- sperm that had not undergone capacitation exhibited normal motility but could not achieve hyperactivated motility needed to traverse the female genital tract. Ultrastructure of the motility apparatus in Pmca4 -/- sperm tails was normal, but an increased incidence of mitochondrial condensation indicated Ca2+ overload. Immunoblotting and immuno-histochemistry showed that PMCA4 is the most abundant isoform in testis and sperm and that it is localized to the principle piece of the sperm tail, which is also the location of the major Ca2+ channel (CatSper) required for sperm motility. These results are consistent with an essential housekeeping or developmental function for PMCA1, but not PMCA4, and show that PMCA4 expression in the principle piece of the sperm tail is essential for hyperactivated motility and male fertility.

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