Targeted disruption of the Fgf2 gene does not affect vascular growth in the mouse ischemic hindlimb

Chris J. Sullivan, Thomas C Doetschman, James B. Hoying

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Ischemic revascularization involves extensive structural adaptation of the vasculature, including both angiogenesis and arteriogenesis. Previous studies suggest that fibroblast growth factor (FGF)-2 participates in both angiogenesis and arteriogenesis. Despite this, the specific role of endogenous FGF-2 in vascular adaptation during ischemic revascularization is unknown. Therefore, we used femoral artery ligation in Fgf2+/+ and Fgf2-/- mice to test the hypothesis that endogenous FGF-2 is an important regulator of angiogenesis and arteriogenesis in the setting of hindlimb ischemia. Femoral ligation increased capillary and arteriole density in the ischemic calf in both Fgf2+/+ and Fgf2-/- mice. The level of angiographically visible arteries in the thigh was increased in the ischemic hindlimb in all mice, and no significant differences were observed between Fgf2+/+ and Fgf2-/- mice. Additionally, limb perfusion progressively improved to peak values at day 35 postsurgery in both genotypes. Given the equivalent responses observed in Fgf2+/+ and Fgf2-/- mice, we demonstrate that endogenous FGF-2 is not required for revascularization in the setting of peripheral ischemia. Vascular adaptation, including both angiogenesis and arteriogenesis, was not affected by the absence of FGF-2 in this model.

Original languageEnglish (US)
Pages (from-to)2009-2017
Number of pages9
JournalJournal of Applied Physiology
Volume93
Issue number6
StatePublished - Dec 2002
Externally publishedYes

Fingerprint

Fibroblast Growth Factor 2
Hindlimb
Blood Vessels
Growth
Genes
Thigh
Ligation
Ischemia
Arterioles
Femoral Artery
Extremities
Arteries
Perfusion
Genotype

Keywords

  • Angiogenesis
  • Arteriogenesis
  • Basic fibroblast growth factor
  • Collateralization
  • Revascularization

ASJC Scopus subject areas

  • Endocrinology
  • Physiology
  • Orthopedics and Sports Medicine
  • Physical Therapy, Sports Therapy and Rehabilitation

Cite this

Targeted disruption of the Fgf2 gene does not affect vascular growth in the mouse ischemic hindlimb. / Sullivan, Chris J.; Doetschman, Thomas C; Hoying, James B.

In: Journal of Applied Physiology, Vol. 93, No. 6, 12.2002, p. 2009-2017.

Research output: Contribution to journalArticle

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