Targeted-microbubble binding selectively to GPIIb IIIa receptors of platelet thrombi

Patricia A. Schumann, Jonathan P. Christiansen, Rachel M. Quigley, Thomas P. McCreery, Robert H. Sweitzer, Evan C Unger, Jonathan R. Lindner, Terry O Matsunaga

Research output: Contribution to journalArticle

179 Citations (Scopus)

Abstract

RATIONALE AND OBJECTIVES. New targeted microbubbles directed to the GPIIb IIIa receptor have been developed. The objective was to determine whether targeting microbubbles to clots would enhance ultrasound imaging. Systematic studies were designed to determine whether in vitro methodology is an acceptable predictor of in vivo efficacy. MATERIALS AND METHODS. Bioconjugate ligands were inserted into lipid-coated membranes of perfluorocarbon gas microbubbles and binding studies performed on activated platelets immobilized on cell culture plates. Targeted microbubble binding to clots in a flow through chamber was also assessed. Finally, microbubble binding studies on arteriolar and venular clots in a mouse cremasteric muscle model were conducted. RESULTS. Binding studies on platelet-immobilized plates demonstrated an affinity for targeted microbubbles versus untargeted microbubbles. Semiquantitative light obscuration techniques helped to measure extent of targeted microbubble binding. Targeted microbubbles similarly bound to platelet clots in the flow model. Finally, studies in the mouse model confirmed binding of targeted microbubbles in both venules and arterioles. CONCLUSION. The use of receptor selective targeted microbubbles improved binding to vascular thrombi in both in vitro and in vivo settings.

Original languageEnglish (US)
Pages (from-to)587-593
Number of pages7
JournalInvestigative Radiology
Volume37
Issue number11
DOIs
StatePublished - Nov 1 2002
Externally publishedYes

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Microbubbles
Platelet Glycoprotein GPIIb-IIIa Complex
Thrombosis
Blood Platelets
Fluorocarbons
Immobilized Cells
Venules
Arterioles
Membrane Lipids
Blood Vessels
Ultrasonography
Cell Culture Techniques
Gases

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging
  • Radiological and Ultrasound Technology

Cite this

Targeted-microbubble binding selectively to GPIIb IIIa receptors of platelet thrombi. / Schumann, Patricia A.; Christiansen, Jonathan P.; Quigley, Rachel M.; McCreery, Thomas P.; Sweitzer, Robert H.; Unger, Evan C; Lindner, Jonathan R.; Matsunaga, Terry O.

In: Investigative Radiology, Vol. 37, No. 11, 01.11.2002, p. 587-593.

Research output: Contribution to journalArticle

Schumann, PA, Christiansen, JP, Quigley, RM, McCreery, TP, Sweitzer, RH, Unger, EC, Lindner, JR & Matsunaga, TO 2002, 'Targeted-microbubble binding selectively to GPIIb IIIa receptors of platelet thrombi', Investigative Radiology, vol. 37, no. 11, pp. 587-593. https://doi.org/10.1097/00004424-200211000-00001
Schumann, Patricia A. ; Christiansen, Jonathan P. ; Quigley, Rachel M. ; McCreery, Thomas P. ; Sweitzer, Robert H. ; Unger, Evan C ; Lindner, Jonathan R. ; Matsunaga, Terry O. / Targeted-microbubble binding selectively to GPIIb IIIa receptors of platelet thrombi. In: Investigative Radiology. 2002 ; Vol. 37, No. 11. pp. 587-593.
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