Targeted mutagenesis of the phospholipase D gene results in decreased virulence of Corynebacterium pseudotuberculosis

Peter J. McNamara, Gregory A Bradley, J. Glenn Songer

Research output: Contribution to journalArticle

83 Citations (Scopus)

Abstract

The chromosomal gene encoding the phospholipase D from Corynebacterium pseudotuberculosis (biovar ovis) isolate Whetten 1 was replaced with an allele containing a nonsense mutation. The virulence of the mutant strain (W1.31r1) and the isogenic parental strain were then compared by inoculation of goats. The wild-type strain caused abscessation at the site of infection, which then spread to the regional lymph node, while W1.31r1 had a reduced ability to establish a primary infection and was incapable of dissemination. Our results confirm that phospholipase D is a virulence determinant of C. pseudotuberculosis that increases the persistence and spread of the bacteria within the host.

Original languageEnglish (US)
Pages (from-to)921-930
Number of pages10
JournalMolecular Microbiology
Volume12
Issue number6
DOIs
StatePublished - Jun 1994

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Corynebacterium pseudotuberculosis
Phospholipase D
Mutagenesis
Virulence
Nonsense Codon
Infection
Goats
Genes
Sheep
Lymph Nodes
Alleles
Bacteria

ASJC Scopus subject areas

  • Molecular Biology
  • Microbiology

Cite this

Targeted mutagenesis of the phospholipase D gene results in decreased virulence of Corynebacterium pseudotuberculosis. / McNamara, Peter J.; Bradley, Gregory A; Songer, J. Glenn.

In: Molecular Microbiology, Vol. 12, No. 6, 06.1994, p. 921-930.

Research output: Contribution to journalArticle

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