Targeted mutagenesis of the phospholipase D gene results in decreased virulence of Corynebacterium pseudotuberculosis

Peter J. McNamara, Gregory A. Bradley, J. Glenn Songer

Research output: Contribution to journalArticle

83 Scopus citations

Abstract

The chromosomal gene encoding the phospholipase D from Corynebacterium pseudotuberculosis (biovar ovis) isolate Whetten 1 was replaced with an allele containing a nonsense mutation. The virulence of the mutant strain (W1.31r1) and the isogenic parental strain were then compared by inoculation of goats. The with‐type strain caused abscessation at the site of infection, which then spread to the regional lymph node, while W1.31r1 had a reduced ability to establish a primary infection and was incapable of dissemination. Our results confirm that phospholipase D is a virulence determinant of C. pseudotuberculosis that increases the persistence and spread of the bacteria within the host.

Original languageEnglish (US)
Pages (from-to)921-930
Number of pages10
JournalMolecular Microbiology
Volume12
Issue number6
DOIs
StatePublished - Jun 1994

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

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