Targeting of an intestinal apical endosomal protein to endosomes in nonpolarized cells

Jean Wilson, Tamara L. Colton

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Polarized cells such as epithelial cells and neurons have distinct endosomal compartments associated with different plasma membrane domains. The endosomes of the neuronal cell body and the basolateral cytoplasm of epithelial cells are thought to perform cellular 'housekeeping' functions such as the uptake of nutrients and metabolites, while the endosomes in the apical cytoplasm or axons are thought to be specialized for the sorting and transcytosis of cell type-specific ligands and receptors. However, it is not known if non-polarized cells such as fibroblasts contain a specialized endosomal compartment analogous to the specialized endosomes found in neurons and epithelia. We have expressed a protein that is normally found in the apical early endosomes of developing intestinal epithelial cells in normal rat kidney fibroblasts. This apical endosomal marker, called endotubin, is targeted to early endosomes in transfected fibroblasts, and is present in peripheral as well as perinuclear endosomes. The peripheral endosomes that contain endotubin appear to exclude transferrin, fluid phase markers, and the mannose-6-phosphate receptor, although in the perinuclear region colocalization of endotubin and these markers is present. In addition, endotubin positive structures do not tubulate in response to brefeldin A and instead redistribute to a diffuse perinuclear location. Since this endosomal compartment has many of the characteristics of an apical or axonal endosomal compartment, our results indicate that nonpolarized cells also contain a specialized early endosomal compartment.

Original languageEnglish (US)
Pages (from-to)319-330
Number of pages12
JournalJournal of Cell Biology
Volume136
Issue number2
DOIs
StatePublished - 1997

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Endosomes
Proteins
Fibroblasts
Epithelial Cells
Cytoplasm
IGF Type 2 Receptor
Brefeldin A
Transcytosis
Neurons
Housekeeping
Transferrin
Axons
Epithelium
Cell Membrane
Ligands
Kidney
Food

ASJC Scopus subject areas

  • Cell Biology

Cite this

Targeting of an intestinal apical endosomal protein to endosomes in nonpolarized cells. / Wilson, Jean; Colton, Tamara L.

In: Journal of Cell Biology, Vol. 136, No. 2, 1997, p. 319-330.

Research output: Contribution to journalArticle

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