Targeting the secretory pathway of Toxoplasma gondii

Verena Karsten, Huilin Qi, Con J. Beckers, Keith A. Joiner

Research output: Contribution to journalArticle

23 Scopus citations

Abstract

Little is known about the extent of conservation in the organization of the secretory pathway in organisms as different as prokaryotes, eukaryotes, and humans. The protozoan parasite Toxoplasma gondii allows easy genetic manipulations, and numerous vectors for selection of transgenic parasites have been developed. One approach to study the molecular mechanism of protein sorting and trafficking is the expression of foreign proteins. Here we describe the design and application of a vector that targets proteins to the secretory pathway of T. gondii and yields high-level expression of Escherichia coli reporter proteins. The general strategies and potential problems in expressing foreign proteins in T. gondii are discussed.

Original languageEnglish (US)
Pages (from-to)103-111
Number of pages9
JournalMethods: A Companion to Methods in Enzymology
Volume13
Issue number2
DOIs
StatePublished - Oct 1997
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)

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