Task-based imaging of colon cancer in theApcMin/+ mouse model

James B. McNally, Nathaniel D. Kirkpatrick, Lida P. Hariri, Alexandre R. Tumlinson, David G. Besselsen, Eugene W. Gerner, Urs Utzinger, Jennifer K. Barton

Research output: Contribution to journalArticle

39 Scopus citations

Abstract

Optical coherence tomography (OCT), laser-induced fluorescence (LIF), and laser-scanning confocal microscopy (LSCM) were used for the task of multimodal study of healthy and adenomatous mouse colon. The results from each modality were compared with histology, which served as the gold standard. The Apc Min/+ genetic mouse model of colon cancer was compared with wild-type mice. In addition, a special diet was used for the task of studying the origins of a 680 nm autofluorescent signal that was previously observed in colon. The study found close agreement among each of the modalities and with histology. All four modalities were capable of identifying diseased tissue accurately. The OCT and LSCM images provided complementary structural information about the tissue, while the autofluorescence signal measured by LIF and LSCM provided biochemical information. OCT and LIF were performed in vivo and nondestructively, while the LSCM and histology required extraction of the tissue. The magnitude of the 680 nm signal correlates with chlorophyll content in the mouse diet, suggesting that the autofluorescent compound is a dietary metabolite.

Original languageEnglish (US)
Pages (from-to)3049-3062
Number of pages14
JournalApplied optics
Volume45
Issue number13
DOIs
StatePublished - May 1 2006

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Engineering (miscellaneous)
  • Electrical and Electronic Engineering

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    McNally, J. B., Kirkpatrick, N. D., Hariri, L. P., Tumlinson, A. R., Besselsen, D. G., Gerner, E. W., Utzinger, U., & Barton, J. K. (2006). Task-based imaging of colon cancer in theApcMin/+ mouse model. Applied optics, 45(13), 3049-3062. https://doi.org/10.1364/AO.45.003049