Taura syndrome virus from Belize represents a unique variant

Heidi S. Erickson, Bonnie T. Poulos, Feng-Jyu Tang-Nelson, Deborah Bradley-Dunlop, Donald V Lightner

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

A Taura syndrome virus (TSV) isolate from cultured Penaeus vannamei grown in Belize, Central America was characterized and shown to be a unique isolate. Mortality rates in laboratory infections of specific pathogen-free (SPF) P. vannamei, reactivity of the virus with monoclonal antibody (MAb) 1A1 and phylogenetic analysis demonstrated that the Belize isolate (BLZ02TSV) is a new variant of TSV. The Hawaiian 1994 TSV isolate (HI94TSV, GenBank AF277675) was used as the reference isolate for these studies. Laboratory infections of SPF P. vannamei with BLZ02TSV demonstrated higher mortalities and earlier onset of mortalities compared to infections with HI94TSV Shrimp tissues infected with BLZ02TSV reacted with a TSV-specific gene probe by in situ hybridization and were positive by RT-PCR using TSV diagnostic primers, thus indicating that the isolate was TSV. However, Western blot analysis and immunohistochemistry using MAb 1A1 demonstrated that BLZ02TSV did not react with the antibody, suggestive of changes in the VP1 region of the genome that codes for the polypeptide to which MAb 1A1 binds. Phylogenetic analysis of a 1.3 kbp fragment of the TSV VP1 capsid region revealed that BLZ02TSV represents a distinct group among more than 29 isolates of TSV studied thus far. This research demonstrates that BLZ02TSV is a unique isolate of TSV and reiterates a problem related to the use of MAb 1A1 for detection of TSV in clinical specimens.

Original languageEnglish (US)
Pages (from-to)91-98
Number of pages8
JournalDiseases of Aquatic Organisms
Volume64
Issue number2
StatePublished - Apr 18 2005

Fingerprint

Taura syndrome virus
Belize
virus
antibody
monoclonal antibodies
Litopenaeus vannamei
mortality
infection
pathogen
phylogenetics
capsid
pathogens
phylogeny
antibody detection
Central America
in situ hybridization
immunohistochemistry
polypeptides
Western blotting
shrimp

Keywords

  • Belize
  • Monoclonal antibody 1A1
  • Taura syndrome virus

ASJC Scopus subject areas

  • Aquatic Science
  • Ecology

Cite this

Taura syndrome virus from Belize represents a unique variant. / Erickson, Heidi S.; Poulos, Bonnie T.; Tang-Nelson, Feng-Jyu; Bradley-Dunlop, Deborah; Lightner, Donald V.

In: Diseases of Aquatic Organisms, Vol. 64, No. 2, 18.04.2005, p. 91-98.

Research output: Contribution to journalArticle

Erickson, HS, Poulos, BT, Tang-Nelson, F-J, Bradley-Dunlop, D & Lightner, DV 2005, 'Taura syndrome virus from Belize represents a unique variant', Diseases of Aquatic Organisms, vol. 64, no. 2, pp. 91-98.
Erickson, Heidi S. ; Poulos, Bonnie T. ; Tang-Nelson, Feng-Jyu ; Bradley-Dunlop, Deborah ; Lightner, Donald V. / Taura syndrome virus from Belize represents a unique variant. In: Diseases of Aquatic Organisms. 2005 ; Vol. 64, No. 2. pp. 91-98.
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