Taurine-, aspartate- and glutamate-like immunoreactivity identifies chemically distinct subdivisions of Kenyon cells in the cockroach mushroom body

Irina Sinakevitch, Sarah M. Farris, Nicholas J Strausfeld

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

The lobes of the mushroom bodies of the cockroach Periplaneta americana consist of longitudinal modules called laminae. These comprise repeating arrangements of Kenyon cell axons, which like their dendrites and perikarya have an affinity to one of three antisera: to taurine, aspartate, or glutamate. Taurine-immunopositive laminae alternate with immunonegative ones. Aspartate-immunopositive Kenyon cell axons are distributed across the lobes. However, smaller leaf-like ensembles of axons that reveal particularly high affinities to anti-aspartate are embedded within taurine-positive laminae and occur in the immunonegative laminae between them. Together, these arrangements reveal a complex architecture of repeating subunits whose different levels of immunoreactivity correspond to broader immunoreactive layers identified by sera against the neuromodulator FMRFamide. Throughout development and in the adult, the most posterior lamina is glutamate immunopositive. Its axons arise from the most recently born Kenyon cells that in the adult retain their juvenile character, sending a dense system of collaterals to the front of the lobes. Glutamate-positive processes intersect aspartate- and taurine-immunopositive laminae and are disposed such that they might play important roles in synaptogenesis or synapse modification. Glutamate immunoreactivity is not seen in older, mature axons, indicating that Kenyon cells show plasticity of neurotransmitter phenotype during development. Aspartate may be a universal transmitter substance throughout the lobes. High levels of taurine immunoreactivity occur in broad laminae containing the high concentrations of synaptic vesicles.

Original languageEnglish (US)
Pages (from-to)352-367
Number of pages16
JournalJournal of Comparative Neurology
Volume439
Issue number3
DOIs
StatePublished - Oct 22 2001

Fingerprint

Mushroom Bodies
Cockroaches
Taurine
Aspartic Acid
Axons
Glutamic Acid
Neurotransmitter Agents
FMRFamide
Periplaneta
Synaptic Vesicles
Dendrites
Synapses
Immune Sera
Phenotype
Serum

Keywords

  • Amino acid immunoreactivity
  • Brain parcellation
  • Glutamate
  • Insect
  • Learning and memory

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Taurine-, aspartate- and glutamate-like immunoreactivity identifies chemically distinct subdivisions of Kenyon cells in the cockroach mushroom body. / Sinakevitch, Irina; Farris, Sarah M.; Strausfeld, Nicholas J.

In: Journal of Comparative Neurology, Vol. 439, No. 3, 22.10.2001, p. 352-367.

Research output: Contribution to journalArticle

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