Taurine reabsorption by a carrier interacting with furosemide in short and long Henle's loops of rat nephrons

Stefan Silbernagl, Katharina Völker, Hans J. Lang, William H Dantzler

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Taurine is net reabsorbed in the proximal convolution by Cl- - stimulated Na+ symport specific for β-amine acids but not in later nephron segments. However, large unidirectional taurine transport takes place there. To investigate unidirectional taurine reabsorption, we comicroin-fused [3H]taurine and [14C]inulin into late proximal (LP), early distal (ED), and late distal (LD) tubule segments, as well as into the tips of long loops of Henle (LLH) of rats in vivo, and determined fractional reabsorption of [3H]taurine (FR) in the ipsilateral urine. FR (9 μmol/1 taurine) was 80-93 (LP), 16 (ED), and 8% (LD). At 26 mmol/l taurine, FR decreased to 13 (LP) and 6% (ED). FR also decreased when Na+ or C1- was absent or furosemide (5 x 10-5 mol/l) was added. Bumetanide (5 x 10-5 mol/l) had no effect, whereas aniline-2-sulfonic acid (ASA) inhibited. During LLH microinfusion, FR was 55% at 66 μmol/1 and 17% at 228 μmol/l and was again inhibited by furosemide and ASA but not by bumetanide. [14C]taurine reabsorption from microperfused proximal convolutions was not influenced by furosemide. Chronic water diuresis did not affect taurine reabsorption in short Henle's loops. We conclude that taurine can enter cells of the distal nephron from the lumen by an Na+- and partly C1- -dependent carrier with which C(α,β)-substituted taurine (ASA) and C(α,β-) and N-substituted β-alanine (furosemide) directly interact. Thus proximal and distal taurine carriers seem to be different.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Renal Physiology
Volume272
Issue number2 41-2
StatePublished - Feb 1997

Fingerprint

Loop of Henle
Taurine
Nephrons
Furosemide
Sulfonic Acids
Bumetanide
Inulin
Diuresis
Ion Transport
Alanine
Amines

Keywords

  • β-alanine
  • Aniline-2-sulfonic acid
  • Kidney
  • Organic osmolyte
  • Renal amino acid transport
  • Specificity of taurine carrier

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

Cite this

Taurine reabsorption by a carrier interacting with furosemide in short and long Henle's loops of rat nephrons. / Silbernagl, Stefan; Völker, Katharina; Lang, Hans J.; Dantzler, William H.

In: American Journal of Physiology - Renal Physiology, Vol. 272, No. 2 41-2, 02.1997.

Research output: Contribution to journalArticle

@article{787c13e0f7654359a699310c2827099b,
title = "Taurine reabsorption by a carrier interacting with furosemide in short and long Henle's loops of rat nephrons",
abstract = "Taurine is net reabsorbed in the proximal convolution by Cl- - stimulated Na+ symport specific for β-amine acids but not in later nephron segments. However, large unidirectional taurine transport takes place there. To investigate unidirectional taurine reabsorption, we comicroin-fused [3H]taurine and [14C]inulin into late proximal (LP), early distal (ED), and late distal (LD) tubule segments, as well as into the tips of long loops of Henle (LLH) of rats in vivo, and determined fractional reabsorption of [3H]taurine (FR) in the ipsilateral urine. FR (9 μmol/1 taurine) was 80-93 (LP), 16 (ED), and 8{\%} (LD). At 26 mmol/l taurine, FR decreased to 13 (LP) and 6{\%} (ED). FR also decreased when Na+ or C1- was absent or furosemide (5 x 10-5 mol/l) was added. Bumetanide (5 x 10-5 mol/l) had no effect, whereas aniline-2-sulfonic acid (ASA) inhibited. During LLH microinfusion, FR was 55{\%} at 66 μmol/1 and 17{\%} at 228 μmol/l and was again inhibited by furosemide and ASA but not by bumetanide. [14C]taurine reabsorption from microperfused proximal convolutions was not influenced by furosemide. Chronic water diuresis did not affect taurine reabsorption in short Henle's loops. We conclude that taurine can enter cells of the distal nephron from the lumen by an Na+- and partly C1- -dependent carrier with which C(α,β)-substituted taurine (ASA) and C(α,β-) and N-substituted β-alanine (furosemide) directly interact. Thus proximal and distal taurine carriers seem to be different.",
keywords = "β-alanine, Aniline-2-sulfonic acid, Kidney, Organic osmolyte, Renal amino acid transport, Specificity of taurine carrier",
author = "Stefan Silbernagl and Katharina V{\"o}lker and Lang, {Hans J.} and Dantzler, {William H}",
year = "1997",
month = "2",
language = "English (US)",
volume = "272",
journal = "American Journal of Physiology",
issn = "0363-6143",
publisher = "American Physiological Society",
number = "2 41-2",

}

TY - JOUR

T1 - Taurine reabsorption by a carrier interacting with furosemide in short and long Henle's loops of rat nephrons

AU - Silbernagl, Stefan

AU - Völker, Katharina

AU - Lang, Hans J.

AU - Dantzler, William H

PY - 1997/2

Y1 - 1997/2

N2 - Taurine is net reabsorbed in the proximal convolution by Cl- - stimulated Na+ symport specific for β-amine acids but not in later nephron segments. However, large unidirectional taurine transport takes place there. To investigate unidirectional taurine reabsorption, we comicroin-fused [3H]taurine and [14C]inulin into late proximal (LP), early distal (ED), and late distal (LD) tubule segments, as well as into the tips of long loops of Henle (LLH) of rats in vivo, and determined fractional reabsorption of [3H]taurine (FR) in the ipsilateral urine. FR (9 μmol/1 taurine) was 80-93 (LP), 16 (ED), and 8% (LD). At 26 mmol/l taurine, FR decreased to 13 (LP) and 6% (ED). FR also decreased when Na+ or C1- was absent or furosemide (5 x 10-5 mol/l) was added. Bumetanide (5 x 10-5 mol/l) had no effect, whereas aniline-2-sulfonic acid (ASA) inhibited. During LLH microinfusion, FR was 55% at 66 μmol/1 and 17% at 228 μmol/l and was again inhibited by furosemide and ASA but not by bumetanide. [14C]taurine reabsorption from microperfused proximal convolutions was not influenced by furosemide. Chronic water diuresis did not affect taurine reabsorption in short Henle's loops. We conclude that taurine can enter cells of the distal nephron from the lumen by an Na+- and partly C1- -dependent carrier with which C(α,β)-substituted taurine (ASA) and C(α,β-) and N-substituted β-alanine (furosemide) directly interact. Thus proximal and distal taurine carriers seem to be different.

AB - Taurine is net reabsorbed in the proximal convolution by Cl- - stimulated Na+ symport specific for β-amine acids but not in later nephron segments. However, large unidirectional taurine transport takes place there. To investigate unidirectional taurine reabsorption, we comicroin-fused [3H]taurine and [14C]inulin into late proximal (LP), early distal (ED), and late distal (LD) tubule segments, as well as into the tips of long loops of Henle (LLH) of rats in vivo, and determined fractional reabsorption of [3H]taurine (FR) in the ipsilateral urine. FR (9 μmol/1 taurine) was 80-93 (LP), 16 (ED), and 8% (LD). At 26 mmol/l taurine, FR decreased to 13 (LP) and 6% (ED). FR also decreased when Na+ or C1- was absent or furosemide (5 x 10-5 mol/l) was added. Bumetanide (5 x 10-5 mol/l) had no effect, whereas aniline-2-sulfonic acid (ASA) inhibited. During LLH microinfusion, FR was 55% at 66 μmol/1 and 17% at 228 μmol/l and was again inhibited by furosemide and ASA but not by bumetanide. [14C]taurine reabsorption from microperfused proximal convolutions was not influenced by furosemide. Chronic water diuresis did not affect taurine reabsorption in short Henle's loops. We conclude that taurine can enter cells of the distal nephron from the lumen by an Na+- and partly C1- -dependent carrier with which C(α,β)-substituted taurine (ASA) and C(α,β-) and N-substituted β-alanine (furosemide) directly interact. Thus proximal and distal taurine carriers seem to be different.

KW - β-alanine

KW - Aniline-2-sulfonic acid

KW - Kidney

KW - Organic osmolyte

KW - Renal amino acid transport

KW - Specificity of taurine carrier

UR - http://www.scopus.com/inward/record.url?scp=0030764261&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030764261&partnerID=8YFLogxK

M3 - Article

C2 - 9124397

AN - SCOPUS:0030764261

VL - 272

JO - American Journal of Physiology

JF - American Journal of Physiology

SN - 0363-6143

IS - 2 41-2

ER -