TCRβ enhancer activation in early and late lymphoid progenitors

Hillary H. Norris, Aaron J. Martin, Lonnie P. Lybarger, Hanne Andersen, Deborah C. Chervenak, Robert Chervenak

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

An earlier report from our laboratory indicates that the activation of the T cell receptor (TCR) β enhancer (Eβ) is not always an indicator of T lineage potential in bone marrow-resident pre-lymphocytes. In order to more precisely investigate the consequences of Eβ activation in lymphopoiesis, a genetic reporter animal, in which the expression of green fluorescent protein (GFP) is controlled by Eβ, was used to examine two well-defined lymphopotent populations. Adoptive transfer experiments suggest that primitive lymphoid precursor populations (specifically, hematopoietic stem cells) consist of two discrete-populations discernible by Eβ-GFP activation, although the two populations display no overt differences in lineage potential. In contrast, subsets of more differentiated pre-lymphocytes (specifically, common lymphoid progenitors), while also discernible by Eβ-GFP activation, display different capacities for reconstituting lymphoid compartments. Interestingly, late lymphoid progenitors containing inactive Eβ elements generated both T and B cells in vivo, in accord with the original description of this population; however, progenitors containing active Eβ elements displayed an unexpected bias toward the B lineage. Our findings suggest that Eβ activation is an indicator of B lineage specification in late, but not early lymphoid precursors.

Original languageEnglish (US)
Pages (from-to)59-71
Number of pages13
JournalCellular Immunology
Volume247
Issue number2
DOIs
StatePublished - Jun 1 2007
Externally publishedYes

Keywords

  • Bone marrow
  • Common lymphoid progenitor
  • Hematopoietic stem cell
  • Lymphoid precursor
  • Lymphopoiesis
  • T cell receptor β chain

ASJC Scopus subject areas

  • Immunology

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