TCRβ enhancer activation in early and late lymphoid progenitors

Hillary H. Norris, Aaron J. Martin, Lonnie Lybarger, Hanne Andersen, Deborah C. Chervenak, Robert Chervenak

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

An earlier report from our laboratory indicates that the activation of the T cell receptor (TCR) β enhancer (Eβ) is not always an indicator of T lineage potential in bone marrow-resident pre-lymphocytes. In order to more precisely investigate the consequences of Eβ activation in lymphopoiesis, a genetic reporter animal, in which the expression of green fluorescent protein (GFP) is controlled by Eβ, was used to examine two well-defined lymphopotent populations. Adoptive transfer experiments suggest that primitive lymphoid precursor populations (specifically, hematopoietic stem cells) consist of two discrete-populations discernible by Eβ-GFP activation, although the two populations display no overt differences in lineage potential. In contrast, subsets of more differentiated pre-lymphocytes (specifically, common lymphoid progenitors), while also discernible by Eβ-GFP activation, display different capacities for reconstituting lymphoid compartments. Interestingly, late lymphoid progenitors containing inactive Eβ elements generated both T and B cells in vivo, in accord with the original description of this population; however, progenitors containing active Eβ elements displayed an unexpected bias toward the B lineage. Our findings suggest that Eβ activation is an indicator of B lineage specification in late, but not early lymphoid precursors.

Original languageEnglish (US)
Pages (from-to)59-71
Number of pages13
JournalCellular Immunology
Volume247
Issue number2
DOIs
StatePublished - Jun 2007
Externally publishedYes

Fingerprint

T-Cell Antigen Receptor
Green Fluorescent Proteins
Population
Lymphoid Progenitor Cells
Lymphocytes
Lymphopoiesis
Adoptive Transfer
Hematopoietic Stem Cells
B-Lymphocytes
Bone Marrow
T-Lymphocytes

Keywords

  • Bone marrow
  • Common lymphoid progenitor
  • Hematopoietic stem cell
  • Lymphoid precursor
  • Lymphopoiesis
  • T cell receptor β chain

ASJC Scopus subject areas

  • Cell Biology
  • Immunology

Cite this

Norris, H. H., Martin, A. J., Lybarger, L., Andersen, H., Chervenak, D. C., & Chervenak, R. (2007). TCRβ enhancer activation in early and late lymphoid progenitors. Cellular Immunology, 247(2), 59-71. https://doi.org/10.1016/j.cellimm.2007.04.007

TCRβ enhancer activation in early and late lymphoid progenitors. / Norris, Hillary H.; Martin, Aaron J.; Lybarger, Lonnie; Andersen, Hanne; Chervenak, Deborah C.; Chervenak, Robert.

In: Cellular Immunology, Vol. 247, No. 2, 06.2007, p. 59-71.

Research output: Contribution to journalArticle

Norris, HH, Martin, AJ, Lybarger, L, Andersen, H, Chervenak, DC & Chervenak, R 2007, 'TCRβ enhancer activation in early and late lymphoid progenitors', Cellular Immunology, vol. 247, no. 2, pp. 59-71. https://doi.org/10.1016/j.cellimm.2007.04.007
Norris, Hillary H. ; Martin, Aaron J. ; Lybarger, Lonnie ; Andersen, Hanne ; Chervenak, Deborah C. ; Chervenak, Robert. / TCRβ enhancer activation in early and late lymphoid progenitors. In: Cellular Immunology. 2007 ; Vol. 247, No. 2. pp. 59-71.
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